Name: MitoDNA™ Blue 470
Brand: AAT Bioquest
SKU: 22684
Price: 301 USD
Availability: InStock

MitoDNA™ Blue 470

MitoDNA™ Blue 470 is a mitochondrial DNA-selective fluorescent probe that specifically accumulates in mitochondria and binds mtDNA, overcoming the nuclear bias of conventional DNA stains like DAPI.

mtDNA-Specific: Selectively binds to mtDNA without nuclear interference
Live-Cell Compatibility: Easily permeates cells for real-time mtDNA tracking
High Signal Clarity: Large Stokes shift ensures a strong signal-to-noise ratio for multiplex imaging
Broad Research Applicability: Ideal for investigating mtDNA-linked diseases and mitochondrial function

Fluorescence response of MitoDNA™ Blue 470 (5 µM) before and after DNase (2 units/reaction) at 37 °C for 1 hour treatment in HeLa cells. The fluorescence intensities were monitored with fluorescence microscopy.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22684	1 mg	Price

Overview

There are very few probes that can be effectively used to detect mitochondrial deoxyribonucleic acid (mtDNA). The common fluorescent DNA probes (such as DAPI, Hoechst or SYBR® Green) lack the specificity to target mitochondria. They predominantly stain nuclei. MitoDNA™ Blue 470 is a cell permeable dye that specifically stains mtDNA in live cells. It provides an efficient way of labeling mtDNA for the dynamic imaging of mtDNA in live cells. MitoDNA™ Blue 470 has a good Stokes Shift that gives great signal-to-noise ratio and can be easily used for multiplex staining in cells with other fluorescent imaging probes. mtDNA is a small circular DNA found within mitochondria present in the cytoplasm of a cell. This DNA is supplementary to the nucleic acid material found in the nucleus of each cell. The mtDNA codes for 37 genes that promote the proper functioning of some cells. The mitochondria synthesize adenosine triphosphate (ATP) through oxidative phosphorylation and encode information for the synthesis of enzymes, transfer ribonucleic acid (tRNA), and ribosomal RNA (rRNA). Disorders of mtDNA and mutations in its genes can predispose to health problems like age-related hearing loss, diabetes, and brain, heart, and liver failure, among other conditions. Moreover, mtDNA and its associated mitochondrial disorders can predispose people to different types of cancers including lymphomas, leukemias, and breast, intestine, liver and kidney tumors etc.

References

View all 50 references: Citation Explorer

Mature oocyte dysmorphisms may be associated with progesterone levels, mitochondrial DNA content, and vitality in luteal granulosa cells.

Authors:

Raad, Georges and Tanios, Judy and Serdarogullari, Munevver and Bazzi, Marwa and Mourad, Youmna and Azoury, Joseph and Yarkiner, Zalihe and Liperis, Georgios and Fakih, Fadi and Fakih, Chadi

Journal:

Journal of assisted reproduction and genetics (2024): 795-813

Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs.

Authors:

Llavanera, Marc and Mateo-Otero, Yentel and Viñolas-Vergés, Estel and Bonet, Sergi and Yeste, Marc

Journal:

Journal of animal science and biotechnology (2024): 10

Mitochondrial DNA content and methylation in sperm of patients with asthenozoospermia.

Authors:

Geng, Qiang and Gao, Ruifang and Sun, Yuan and Chen, Shaofeng and Sun, Lili and Li, Wei and Li, Zhong and Zhao, Yu and Zhao, Feng and Zhang, Ying and Li, Anwen and Liu, Hongbin

Journal:

Journal of assisted reproduction and genetics (2024)

Early life exposure to mercury and relationships with telomere length and mitochondrial DNA content in European children.

Authors:

Lozano, Manuel and McEachan, Rosemary R C and Wright, John and Yang, Tiffany C and Dow, Courtney and Kadawathagedara, Manik and Lepeule, Johanna and Bustamante, Mariona and Maitre, Lea and Vrijheid, Martine and Brantsæter, Anne Lise and Meltzer, Helle Margrete and Bempi, Vasiliki and Roumeliotaki, Theano and Thomsen, Cathrine and Nawrot, Tim and Broberg, Karin and Llop, Sabrina

Journal:

The Science of the total environment (2024): 173014

Clinical significance of mitochondrial DNA content in acute promyelocytic leukaemia.

Authors:

Pereira-Martins, Diego A and Coelho-Silva, Juan L and Weinhäuser, Isabel and Franca-Neto, Pedro L and Silveira, Douglas R and Ortiz, César and Moreira-Aguiar, Amanda and Lima, Marinus M and Koury, Luisa C and de Melo, Raul A and Glória, Ana B and Fagundes, Evandro M and Lino, Bruno K and Pagnano, Katia and Bittencourt, Rosane and Nunes, Elenaide and Traina, Fabiola and Figueiredo-Pontes, Lorena and Keating, Armand and Tallman, Martin S and Ribeiro, Raul C and Dilon, Richard and Ganser, Arnold and Sanz, Miguel A and Berliner, Nancy and Valk, Peter and Löwenberg, Bob and Ottone, Tiziana and Noguera, Nelida I and Voso, Maria T and Paoloni, Francesca and Fazi, Paola and Ammatuna, Emanuele and Huls, Gerwin and Schuringa, Jan Jacob and Rego, Eduardo M and Lucena-Araujo, Antonio R

Journal:

British journal of haematology (2023): 170-174

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	344
Emission (nm)	469

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	DAPI Filter
Emission	DAPI Filter
Recommended plate	Black wall/clear bottom

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Page updated on October 14, 2025