Name: MitoDNA™ Green 530
Brand: AAT Bioquest
SKU: 22685
Price: 308 USD
Availability: InStock

MitoDNA™ Green 530

MitoDNA™ Green 530 is a green-fluorescent mitochondrial nucleoid stain that selectively labels mtDNA without nuclear interference, enabling specific visualization of mitochondrial DNA in live cells.

mtDNA-Specific: Selectively binds to mtDNA without nuclear interference
Live-Cell Compatibility: Easily permeates cells for real-time mtDNA tracking
High Signal Clarity: Large Stokes shift ensures a strong signal-to-noise ratio for multiplex imaging
Broad Research Applicability: Ideal for investigating mtDNA-linked diseases and mitochondrial function

Fluorescence response of MitoDNA™ Green 530 (0.2 µM) in HeLa cells before and after treatment with DNase (2 units/reaction) at 37ºC for 1 hour. Fluorescence intensities were monitored using fluorescence microscopy.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22685	1 mg	Price

Overview

Detecting mitochondrial DNA (mtDNA) has been challenging due to the non-specific nature of conventional DNA probes like DAPI, Hoechst, or SYBR® Green, which primarily stain the nucleus. MitoDNA™ Green 530 overcomes this limitation with its high specificity for mtDNA, allowing researchers to selectively visualize and track mitochondrial dynamics in live cells. This cell-permeable dye offers a large Stokes Shift, providing a high signal-to-noise ratio ideal for multiplex imaging alongside other fluorescent probes.

mtDNA is a small, circular DNA found in the mitochondria of a cell, separate from the nuclear DNA. It encodes 37 genes essential for mitochondrial function, including those involved in ATP synthesis, enzyme production, and tRNA and rRNA synthesis. Mutations or disorders in mtDNA can lead to various health issues, including age-related hearing loss, diabetes, and failures of the brain, heart, and liver. Additionally, mtDNA mutations are linked to an increased risk of cancers, such as lymphomas, leukemias, and tumors in the breast, intestine, liver, and kidneys

References

View all 43 references: Citation Explorer

Classification, identification, and DNA barcoding study for common cockroach species (Dictyoptera: Blattaria) from China.

Authors:

Li, Hu and Shangqing, Zhang and Yae, Zhao and Fan, Yang and Xinyue, Zhang and Shirui, Liu and Tianyi, Zhang and Dongling, Niu

Journal:

Gene (2025): 148981

Molecular phylodiagnosis of Echinococcus multilocularis and Echinococcus granulosus among canids in Guilan province, northern Iran.

Authors:

Feiz-Haddad, Mohammad Hossein and Moradkhani, Mohammad-Ali

Journal:

Comparative immunology, microbiology and infectious diseases (2024): 102210

Corrigendum: A revision of the trichostrongylid nematode Cooperia Ransom, 1907, from deer game: recent integrative research confirms the existence of the ancient host-specific species Cooperia ventricosa (Rudolphi, 1809).

Authors:

Albrechtová, Martina and Kašparová, Eva Štefková and Langrová, Iva and Hart, Vlastimil and Neuhaus, Birger and Jankovská, Ivana and Petrtýl, Miroslav and Magdálek, Jan and Špakulová, Marta

Journal:

Frontiers in veterinary science (2024): 1388292

Guanylate Kinase 1 Deficiency: A Novel and Potentially Treatable Mitochondrial DNA Depletion/Deletions Disease.

Authors:

Hidalgo-Gutierrez, Agustin and Shintaku, Jonathan and Ramon, Javier and Barriocanal-Casado, Eliana and Pesini, Alba and Saneto, Russell P and Garrabou, Gloria and Milisenda, Jose Cesar and Matas-Garcia, Ana and Gort, Laura and Ugarteburu, Olatz and Gu, Yue and Koganti, Lahari and Wang, Tian and Tadesse, Saba and Meneri, Megi and Sciacco, Monica and Wang, Shuang and Tanji, Kurenai and Horwitz, Marshall S and Dorschner, Michael O and Mansukhani, Mahesh and Comi, Giacomo Pietro and Ronchi, Dario and Marti, Ramon and Ribes, Antonia and Tort, Frederic and Hirano, Michio

Journal:

Annals of neurology (2024)

The answer, my friend, is blowin' in the wind: Blow sampling provides a new dimension to whale population monitoring.

Authors:

Valsecchi, Elena

Journal:

Molecular ecology resources (2024): e14012

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	421
Emission (nm)	526

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC Filter
Emission	FITC Filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 10, 2026