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MitoDNA™ Green 530

Product key features

  • mtDNA-Specific: Selectively binds to mtDNA without nuclear interference
  • Live-Cell Compatibility: Easily permeates cells for real-time mtDNA tracking
  • High Signal Clarity: Large Stokes shift ensures a strong signal-to-noise ratio for multiplex imaging
  • Broad Research Applicability: Ideal for investigating mtDNA-linked diseases and mitochondrial function

Product description

Detecting mitochondrial DNA (mtDNA) has been challenging due to the non-specific nature of conventional DNA probes like DAPI, Hoechst, or SYBR® Green, which primarily stain the nucleus. MitoDNA™ Green 530 overcomes this limitation with its high specificity for mtDNA, allowing researchers to selectively visualize and track mitochondrial dynamics in live cells. This cell-permeable dye offers a large Stokes Shift, providing a high signal-to-noise ratio ideal for multiplex imaging alongside other fluorescent probes.

mtDNA is a small, circular DNA found in the mitochondria of a cell, separate from the nuclear DNA. It encodes 37 genes essential for mitochondrial function, including those involved in ATP synthesis, enzyme production, and tRNA and rRNA synthesis. Mutations or disorders in mtDNA can lead to various health issues, including age-related hearing loss, diabetes, and failures of the brain, heart, and liver. Additionally, mtDNA mutations are linked to an increased risk of cancers, such as lymphomas, leukemias, and tumors in the breast, intestine, liver, and kidneys

Example protocol

AT A GLANCE

Important Note

Before initial use, thaw MitoDNA™ Green 530 at room temperature and briefly centrifuge to collect the dried pellet.

Protocol Summary
  1. Prepare cells in growth medium.

  2. Stain cells with MitoDNA™ Green 530 working solution.

  3. Incubate samples at 37°C for 5–15 minutes.

  4. Monitor fluorescence intensity with FITC filter.

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

MitoDNA™ Green 530 Stock Solution
  1. Prepare a 5–10 mM MitoDNA™ Green 530 stock solution in DMSO. For example, add 236 μL of DMSO to the MitoDNA™ Green 530 vial to create a 10 mM stock solution.

    Note: Prepare single-use aliquots of the stock solution and store at ≤ -20°C. Protect from light and avoid repeated freeze-thaw cycles.

PREPARATION OF WORKING SOLUTION

MitoDNA™ Green 530 Working Solution
  1. Prepare a 5–10 μM working solution by diluting the MitoDNA™ Green 530 stock solution in Hanks solution with 20 mM Hepes buffer (HHBS, AAT cat# 20011).

    Note: For optimal results, use this solution within a few hours of preparation.

    Note: Protect the working solution from light by covering it with foil or placing it in the dark.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Plate cells as needed in a 96-well black wall, clear bottom plate.

  2. Remove the cell culture medium and add 100 µL of MitoDNA™ Green 530 working solution to each well.

  3. Incubate cells at 37°C in a 5% CO2 incubator for 5–15 minutes, keeping them protected from light.

    Note: The optimal concentration and incubation time may vary by cell line; we recommend testing different concentrations.

  4. Remove the dye working solution and wash the cells twice with HHBS buffer.

  5. Add HHBS buffer and analyze cells using a fluorescence microscope with a FITC filter set.

Spectrum

References

View all 43 references: Citation Explorer
Classification, identification, and DNA barcoding study for common cockroach species (Dictyoptera: Blattaria) from China.
Authors: Li, Hu and Shangqing, Zhang and Yae, Zhao and Fan, Yang and Xinyue, Zhang and Shirui, Liu and Tianyi, Zhang and Dongling, Niu
Journal: Gene (2025): 148981
Molecular phylodiagnosis of Echinococcus multilocularis and Echinococcus granulosus among canids in Guilan province, northern Iran.
Authors: Feiz-Haddad, Mohammad Hossein and Moradkhani, Mohammad-Ali
Journal: Comparative immunology, microbiology and infectious diseases (2024): 102210
Corrigendum: A revision of the trichostrongylid nematode Cooperia Ransom, 1907, from deer game: recent integrative research confirms the existence of the ancient host-specific species Cooperia ventricosa (Rudolphi, 1809).
Authors: Albrechtová, Martina and Kašparová, Eva Štefková and Langrová, Iva and Hart, Vlastimil and Neuhaus, Birger and Jankovská, Ivana and Petrtýl, Miroslav and Magdálek, Jan and Špakulová, Marta
Journal: Frontiers in veterinary science (2024): 1388292
Guanylate Kinase 1 Deficiency: A Novel and Potentially Treatable Mitochondrial DNA Depletion/Deletions Disease.
Authors: Hidalgo-Gutierrez, Agustin and Shintaku, Jonathan and Ramon, Javier and Barriocanal-Casado, Eliana and Pesini, Alba and Saneto, Russell P and Garrabou, Gloria and Milisenda, Jose Cesar and Matas-Garcia, Ana and Gort, Laura and Ugarteburu, Olatz and Gu, Yue and Koganti, Lahari and Wang, Tian and Tadesse, Saba and Meneri, Megi and Sciacco, Monica and Wang, Shuang and Tanji, Kurenai and Horwitz, Marshall S and Dorschner, Michael O and Mansukhani, Mahesh and Comi, Giacomo Pietro and Ronchi, Dario and Marti, Ramon and Ribes, Antonia and Tort, Frederic and Hirano, Michio
Journal: Annals of neurology (2024)
The answer, my friend, is blowin' in the wind: Blow sampling provides a new dimension to whale population monitoring.
Authors: Valsecchi, Elena
Journal: Molecular ecology resources (2024): e14012
Page updated on December 13, 2024

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Catalog Number22685
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Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)

421

Emission (nm)

526

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

Fluorescence microscope

ExcitationFITC Filter
EmissionFITC Filter
Recommended plateBlack wall, clear bottom
Fluorescence response of MitoDNA™ Green 530 (5 µM) in HeLa cells before and after treatment with DNase (2 units/reaction) at 37ºC for 1 hour. Fluorescence intensities were monitored using fluorescence microscopy.
Fluorescence response of MitoDNA™ Green 530 (5 µM) in HeLa cells before and after treatment with DNase (2 units/reaction) at 37ºC for 1 hour. Fluorescence intensities were monitored using fluorescence microscopy.
Fluorescence response of MitoDNA™ Green 530 (5 µM) in HeLa cells before and after treatment with DNase (2 units/reaction) at 37ºC for 1 hour. Fluorescence intensities were monitored using fluorescence microscopy.