MitoLite™ Green FM

MitoLite™ Green FM is a green fluorescent mitochondrial stain for live-cell labeling of mitochondria with reduced dependence on mitochondrial membrane potential.

Live-cell mitochondrial staining: Labels mitochondria in live cells for fluorescence microscopy analysis
Reduced membrane potential dependence: Localizes to mitochondria with less dependence on mitochondrial membrane potential than many potential-sensitive mitochondrial probes
Green fluorescence: Provides a green fluorescent signal suitable for common FITC/GFP imaging channels
Mitochondrial morphology analysis: Supports visualization of mitochondrial structure, distribution, and network organization in live cells

Time-lapse super-resolution fluorescence imaging of live COS-7 cells stained with MitoLite™ Green FM and LysoBrite™ Red. Live COS-7 cells were labeled with MitoLite™ Green FM (Cat. #22695) for mitochondrial staining and LysoBrite™ Red (Cat. #22645) for lysosomal staining. Images were acquired using the CSR Biotech MI-SIM super-resolution microscopy system and reconstructed using standard Wiener reconstruction. The merged sequence shows mitochondria in green and lysosomes in red.

Data credit: Data was generated by CSR Biotech using their MI-SIM microscopy system with MitoLite™ Green FM and LysoBrite™ Red from AAT Bioquest.

Protocol

SDS

Catalog	Size	Price	Quantity
22695	10x50 ug	Price

Overview

MitoLite™ Green FM is a green fluorescent mitochondrial stain designed for live-cell labeling of mitochondria. Unlike many mitochondrial dyes that rely strongly on mitochondrial membrane potential for accumulation, MitoLite™ Green FM shows mitochondrial localization with reduced dependence on membrane potential, making it useful for live-cell mitochondrial imaging workflows.

MitoLite™ Green FM uses the same dye molecule as Thermo Fisher’s MitoTracker Green FM and provides a comparable green-fluorescent mitochondrial staining option. It is suitable for live-cell fluorescence microscopy using common green fluorescence channels; however, it is not well retained after aldehyde fixation and is therefore best used for imaging live cells rather than fixed-cell samples.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MitoLite™ Green FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	148.836 µL	744.181 µL	1.488 mL	7.442 mL	14.884 mL
5 mM	29.767 µL	148.836 µL	297.672 µL	1.488 mL	2.977 mL
10 mM	14.884 µL	74.418 µL	148.836 µL	744.181 µL	1.488 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	671.88 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

3-Hydroxypropionic Acid Enhances Hair Growth-Related Signaling in Human Follicle Dermal Papilla Cells via Activation of the Wnt/$\beta$-Catenin Pathway

Authors:

Jeon, Chae Young and Jo, Yun Hoo and Woo, Seung A and Lee, Yura and Jung, Woochul and Shin, Dong Wook

Journal:

International Journal of Molecular Sciences (2026): 1480

15-Hydroxyprostaglandin Dehydrogenase Inhibitor Restores Endothelial Function Under Dihydrotestosterone-Induced Stress in Human Dermal Microvascular Endothelial Cells

Authors:

Kim, Mujun and Kim, Hak Joong and Lee, Yurim and Lee, Sanghwa and Lim, Dong Chul and Park, Hee Dong and Shin, Dong Wook

Journal:

Molecules (2025): 123

CDK4/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors

Authors:

Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia

Journal:

Oncoimmunology (2022): 2094583

Sonodynamic Therapy Promotes Efferocytosis via CD47 Down-Regulation in Advanced Atherosclerotic Plaque

Authors:

Cao, Yang and Yao, Jianting and Gao, Weiwei and Cao, Zhengyu and Diabakte, Kamal and Wang, Linxin and Sun, Xin and Tian, Ye

Journal:

International Heart Journal (2022): 21--233

References

View all 5 references: Citation Explorer

the influence of platelets and a comparison of analytical approaches

Authors:

Hopkinson, K.; Williams, E. A.; Fairburn, B.; Forster, S.; Flower, D. J.; Saxton, J. M.; Pockley, A. G., A MitoTracker Green-based flow cytometric assay for natural killer cell activity: variability

Journal:

Exp Hematol (2007): 350-7

Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

Authors:

Pendergrass, W.; Wolf, N.; Poot, M.

Journal:

Cytometry A (2004): 162-9

Mitotracker green is a P-glycoprotein substrate

Authors:

Marques-Santos, L. F.; Oliveira, J. G.; Maia, R. C.; Rumjanek, V. M.

Journal:

Biosci Rep (2003): 199-212

MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection

Authors:

Presley, A. D.; Fuller, K. M.; Arriaga, E. A.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 141-50

and MitoTracker Green is affected by mitochondrial membrane potential altering drugs

Authors:

Keij, J. F.; Bell-Prince, C.; Steinkamp, J. A., Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green

Journal:

Cytometry (2000): 203-10

Physical properties

Molecular weight	671.88
Solvent	DMSO

Spectral properties

Excitation (nm)	508
Emission (nm)	528

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	201860-17-5

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 5, 2026