MitoLite™ Green FM

MitoLite™ Green FM is the same molecule to the MitoTracker Green FM (M7514, ThermoFisher). It is green-fluorescent mitochondrial stain. Unlike other MitoLite probes, MitoLite™ Green FM appears to localize to mitochondria, much less depending on mitochondrial membrane potential. The dye stains live cells, but it is not well-retained after aldehyde fixation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22695	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MitoLite™ Green FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	148.836 µL	744.181 µL	1.488 mL	7.442 mL	14.884 mL
5 mM	29.767 µL	148.836 µL	297.672 µL	1.488 mL	2.977 mL
10 mM	14.884 µL	74.418 µL	148.836 µL	744.181 µL	1.488 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	671.88 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

CDK4/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors

Authors:

Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia

Journal:

Oncoimmunology (2022): 2094583

Sonodynamic Therapy Promotes Efferocytosis via CD47 Down-Regulation in Advanced Atherosclerotic Plaque

Authors:

Cao, Yang and Yao, Jianting and Gao, Weiwei and Cao, Zhengyu and Diabakte, Kamal and Wang, Linxin and Sun, Xin and Tian, Ye

Journal:

International Heart Journal (2022): 21--233

References

View all 5 references: Citation Explorer

the influence of platelets and a comparison of analytical approaches

Authors:

Hopkinson, K.; Williams, E. A.; Fairburn, B.; Forster, S.; Flower, D. J.; Saxton, J. M.; Pockley, A. G., A MitoTracker Green-based flow cytometric assay for natural killer cell activity: variability

Journal:

Exp Hematol (2007): 350-7

Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

Authors:

Pendergrass, W.; Wolf, N.; Poot, M.

Journal:

Cytometry A (2004): 162-9

Mitotracker green is a P-glycoprotein substrate

Authors:

Marques-Santos, L. F.; Oliveira, J. G.; Maia, R. C.; Rumjanek, V. M.

Journal:

Biosci Rep (2003): 199-212

MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection

Authors:

Presley, A. D.; Fuller, K. M.; Arriaga, E. A.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 141-50

and MitoTracker Green is affected by mitochondrial membrane potential altering drugs

Authors:

Keij, J. F.; Bell-Prince, C.; Steinkamp, J. A., Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green

Journal:

Cytometry (2000): 203-10

Physical properties

Molecular weight	671.88
Solvent	DMSO

Spectral properties

Excitation (nm)	508
Emission (nm)	528

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	201860-17-5

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 21, 2025