MitoLite™ Red CMXRos

MitoLite™ Red CMXRos is chemically same to the MitoTracker™ Red CMXRos (ThermoFisher). MitoLite™ Red CMXRos is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. MitoLite™ Red CMXRos is well-retained after aldehyde fixation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22698	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MitoLite™ Red CMXRos to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	188.14 µL	940.698 µL	1.881 mL	9.407 mL	18.814 mL
5 mM	37.628 µL	188.14 µL	376.279 µL	1.881 mL	3.763 mL
10 mM	18.814 µL	94.07 µL	188.14 µL	940.698 µL	1.881 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	531.52 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Ros-sensitive hydrogel system incorporating mitochondria-targeted liposomes for periodontitis treatment

Authors:

Liu, Qing and Cong, Rui and Yu, Jinping and Zhang, Xiaoxin and Tu, Jiasheng and Dong, Yingchun and Jiang, Lei and Chen, Bin

Journal:

Materials \& Design (2025): 114557

Tetramerization of PKM2 alleviates traumatic brain injury by ameliorating mitochondrial damage in microglia

Authors:

Zhu, Haiyan and Zhang, Huiwen and Zhao, Xiao-Jing and Zhang, Lingyuan and Liu, Xue and Zhang, Zhi-Yuan and Ren, Yi-Zhi and Feng, Yong

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1$\alpha$ Signaling Pathway

Authors:

Yang, Ziwei and Hong, Wenli and Zheng, K and Feng, Jingyuan and Hu, Chuan and Tan, Jun and Zhong, Zhisheng and Zheng, Yuehui

Journal:

Oxidative Medicine and Cellular Longevity (2022)

Flow cytometric readout based on Mitotracker Red CMXRos staining of live asexual blood stage malarial parasites reliably assesses antibody dependent cellular inhibition

Authors:

Jogd, undefined and , P. S., Singh, S. K., Christiansen, M., Dziegiel, M. H., Singh, S., Theisen, M.

Journal:

Malar J (2012): 235

Compartment-specific Ca2+ imaging in the green alga Chlamydomonas reinhardtii reveals high light-induced chloroplast Ca2+ signatures

Authors:

Pivato, Matteo and Grenzi, Matteo and Costa, Alex and Ballottari, Matteo

Journal:

New Phytologist

References

View all 6 references: Citation Explorer

the influence of platelets and a comparison of analytical approaches

Authors:

Hopkinson, K.; Williams, E. A.; Fairburn, B.; Forster, S.; Flower, D. J.; Saxton, J. M.; Pockley, A. G., A MitoTracker Green-based flow cytometric assay for natural killer cell activity: variability

Journal:

Exp Hematol (2007): 350-7

Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

Authors:

Pendergrass, W.; Wolf, N.; Poot, M.

Journal:

Cytometry A (2004): 162-9

Mitotracker green is a P-glycoprotein substrate

Authors:

Marques-Santos, L. F.; Oliveira, J. G.; Maia, R. C.; Rumjanek, V. M.

Journal:

Biosci Rep (2003): 199-212

MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection

Authors:

Presley, A. D.; Fuller, K. M.; Arriaga, E. A.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 141-50

and MitoTracker Green is affected by mitochondrial membrane potential altering drugs

Authors:

Keij, J. F.; Bell-Prince, C.; Steinkamp, J. A., Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green

Journal:

Cytometry (2000): 203-10

Physical properties

Molecular weight	531.52
Solvent	DMSO

Spectral properties

Excitation (nm)	578
Emission (nm)	598

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	167095-09-2

Instrument settings

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

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Fax
Email	sales@aatbio.com
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Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025