MitoLite™ Red FX600
| Overview |  SDSProtocol |
See also: Mitochondria, Cell Metabolism, Flow Cytometry Reagents, Cell Structures and Organelles, Membrane Potential and Channels
Excitation (nm) 580 | Emission (nm) 598 |
MitoLite™ Red is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Images
Figure 1. Image of U2OS cells stained with MitoLite™ Red FX600 in a Costar black wall/clear bottom 96-well plate.
Figure 2. Cellular Retention of MitoLite™ Red FX600 in HeLa Cells.
Live HeLa cells were stained with MitoLite™ Red FX600 in HH buffer for 30 min at 37oC. Cells were washed for 3 times with HH buffer, and then continually cultured in fresh medium for 2 days. Images right after stain (Day 0), ~24 (Day 1) and 48 hours (Day 2) after stain were taken using fluorescence microscope with a Texas Red® filter set.
Live HeLa cells were stained with MitoLite™ Red FX600 in HH buffer for 30 min at 37oC. Cells were washed for 3 times with HH buffer, and then continually cultured in fresh medium for 2 days. Images right after stain (Day 0), ~24 (Day 1) and 48 hours (Day 2) after stain were taken using fluorescence microscope with a Texas Red® filter set.
Citations
View all 4 citations: Citation Explorer
Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140
Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin
Authors: Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: Apoptosis (2016): 489--501
Authors: Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: Apoptosis (2016): 489--501
Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers
Authors: Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: Journal of medicinal chemistry (2015): 7949--7958
Authors: Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: Journal of medicinal chemistry (2015): 7949--7958
Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes
Authors: Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: Journal of Pineal Research (2015): 267--275
Authors: Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: Journal of Pineal Research (2015): 267--275
References
View all 70 references: Citation Explorer
Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475
Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213
Fluorescence imaging of mitochondria in yeast
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433
A fluorescence assay for peptide translocation into mitochondria
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76
Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007
Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer
Authors: Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: Arch Biochem Biophys (2004): 145
Authors: Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: Arch Biochem Biophys (2004): 145
A fluorescence-based technique for screening compounds that protect against damage to brain mitochondria
Authors: Kristian T, Fiskum G.
Journal: Brain Res Brain Res Protoc (2004): 176
Authors: Kristian T, Fiskum G.
Journal: Brain Res Brain Res Protoc (2004): 176
Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection
Authors: Fuller KM, Duffy CF, Arriaga EA.
Journal: Electrophoresis (2002): 1571
Authors: Fuller KM, Duffy CF, Arriaga EA.
Journal: Electrophoresis (2002): 1571
Fluorescence imaging of metabolic responses in single mitochondria
Authors: Nakayama S, Sakuyama T, Mitaku S, Ohta Y.
Journal: Biochem Biophys Res Commun (2002): 23
Authors: Nakayama S, Sakuyama T, Mitaku S, Ohta Y.
Journal: Biochem Biophys Res Commun (2002): 23
Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
Authors: Dedov VN, Cox GC, Roufogalis BD.
Journal: Micron (2001): 653
Authors: Dedov VN, Cox GC, Roufogalis BD.
Journal: Micron (2001): 653
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Activation of the Mitochondrial Apoptotic Pathway Produces Reactive Oxygen Species and Oxidative Damage in Hepatocytes That Contribute to Liver Tumorigenesis
Calmodulin antagonists induce cell cycle arrest and apoptosis in vitro and inhibit tumor growth in vivo in human multiple myeloma
Cold exposure lowers energy expenditure at the cellular level
Developing high-content assays for hepatoxicity with iPSC-derived cells