MitoLite™ Red FX600

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	580
Emission (nm)	598

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

N/A

Excitation (nm)

580

Emission (nm)

598

MitoLite™ Red is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Spectrum

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Spectral properties

Excitation (nm)	580
Emission (nm)	598

Product Family

Name	Excitation (nm)	Emission (nm)
MitoLite™ Red CMXRos	578	598

Images

Figure 1. Image of U2OS cells stained with MitoLite™ Red FX600 in a Costar black wall/clear bottom 96-well plate.

Figure 2. Cellular Retention of MitoLite™ Red FX600 in HeLa Cells.
Live HeLa cells were stained with MitoLite™ Red FX600 in HH buffer for 30 min at 37oC. Cells were washed for 3 times with HH buffer, and then continually cultured in fresh medium for 2 days. Images right after stain (Day 0), ~24 (Day 1) and 48 hours (Day 2) after stain were taken using fluorescence microscope with a Texas Red® filter set.

Citations

View all 7 citations: Citation Explorer

Quercetin Promotes Apoptosis of Gastric Cancer Cells through the EGFR-ERK Signaling Pathway
Authors: Liu, Yali and Li, Yan and Jiang, Yanjun and Zheng, Xin and Wang, Tianming and Li, Jing and Zhang, Biyun and Zhu, Jiarui and Wei, Xintong and Huang, Ruihua and others,
Journal: Journal of Food Biochemistry (2024)

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1$\alpha$ Signaling Pathway
Authors: Yang, Ziwei and Hong, Wenli and Zheng, K and Feng, Jingyuan and Hu, Chuan and Tan, Jun and Zhong, Zhisheng and Zheng, Yuehui
Journal: Oxidative Medicine and Cellular Longevity (2022)

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
Authors: Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: Colloids and Surfaces B: Biointerfaces (2017): 131--140

Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin
Authors: Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: Apoptosis (2016): 489--501

Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers
Authors: Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: Journal of medicinal chemistry (2015): 7949--7958

Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes
Authors: Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: Journal of Pineal Research (2015): 267--275

Mitochondria morphology and membrane potential under stress conditions
Authors: Ezati, Masoumeh and Zumberg, Inna and Cmiel, Vratislav

References

View all 70 references: Citation Explorer

Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: Feng J, Arriaga EA.
Journal: Electrophoresis (2008): 475

Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: Roy SS, Hajnoczky G.
Journal: Methods (2008): 213

Fluorescence imaging of mitochondria in yeast
Authors: Swayne TC, Gay AC, Pon LA.
Journal: Methods Mol Biol (2007): 433

A fluorescence assay for peptide translocation into mitochondria
Authors: Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: Anal Biochem (2007): 76

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: Duffy CF, MacCraith B, Diamond D, O'Kennedy R, Arriaga EA.
Journal: Lab Chip (2006): 1007

Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer
Authors: Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: Arch Biochem Biophys (2004): 145

A fluorescence-based technique for screening compounds that protect against damage to brain mitochondria
Authors: Kristian T, Fiskum G.
Journal: Brain Res Brain Res Protoc (2004): 176

Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection
Authors: Fuller KM, Duffy CF, Arriaga EA.
Journal: Electrophoresis (2002): 1571

Fluorescence imaging of metabolic responses in single mitochondria
Authors: Nakayama S, Sakuyama T, Mitaku S, Ohta Y.
Journal: Biochem Biophys Res Commun (2002): 23

Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
Authors: Dedov VN, Cox GC, Roufogalis BD.
Journal: Micron (2001): 653