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MitoROS™ 580
Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen, and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and has important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. MitoROS™ 580 is a superoxide-sensitive dye that is localized in mitochondria upon loading into live cells. Oxidation of MitoROS™ 580 by superoxide generates red fluorescence. MitoROS™ 580 can be used to monitor superoxide in mitochondria either with a fluorescence microscope or flow cytometer. MitoROS™ 580 reagent permeates live cells where it selectively targets mitochondria. It is rapidly oxidized by superoxide. It is less likely to be oxidized by other reactive oxygen species (ROS) and reactive nitrogen species (RNS). The oxidized product is highly fluorescent in cells. MitoROS™ 580 provides a valuable tool for investigating oxidative stress in various pathologies. MitoROS™ 580 is equivalent to the MitoSOX™ Mitochondrial Superoxide Indicator (#M36008) that is often used for live-cell imaging (MitoSOX™ is the trademark of ThermoFisher).
Fluorescence response of MitoROS™ 580 (10 µM)to different reactive oxygen species (ROS) and reactive nitrogen species (RNS). The fluorescence intensities were monitored at Ex/Em = 540/590 nm.
Fluorescence response of MitoROS™ 580 (10 µM)to different reactive oxygen species (ROS) and reactive nitrogen species (RNS). The fluorescence intensities were monitored at Ex/Em = 540/590 nm.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
16052500 Tests
Price
 
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)500
Emission (nm)582
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Reactive Oxygen Species (ROS) Detection
Brochure: Fluorescence Cell Imaging Probes & Kits
Flyer: Reactive Oxygen Species (ROS)
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Page updated on October 29, 2025