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MP650 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

ABI 7500 FAST system MP650 dye spectrum.
ABI 7500 FAST system MP650 dye spectrum.
ABI 7500 FAST system MP650 dye spectrum.
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Physical properties
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)639
Emission (nm)653
Quantum yield0.291
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
639
Emission (nm)
653
Quantum yield
0.291
MP650 is designed to have identical spectra to those of Mustang Purple. The MP650 Dye qPCR Calibration Plate is designed for use with the 7500 Real-Time PCR system featuring a Fast 96-well block. It facilitates the calibration and maintenance of your qPCR instruments. To ensure optimal performance, it is recommended to conduct these calibrations biannually. This ready-to-use calibration plate requires no further preparation, streamlining the calibration process. Utilizing this plate may significantly enhance the accuracy of fluorescent spectra representation in multiplex qPCR experiments, leading to improved results. For detailed calibration procedures, please consult the user manual of your specific instrument.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)639
Emission (nm)653
Quantum yield0.291

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References


View all 2 references: Citation Explorer
Quantitative PCR overestimation of DNA in samples contaminated with tin.
Authors: Bonsu, Dan Nana Osei and Higgins, Denice and Simon, Claire and Goodwin, Corey S and Henry, Julianne M and Austin, Jeremy J
Journal: Journal of forensic sciences (2023): 1302-1309
The effects of dithiothreitol (DTT) on fluorescent qPCR dyes.
Authors: Hudson, Brittany C and Cox, Jordan O and Seashols-Williams, Sarah J and Dawson Cruz, Tracey
Journal: Journal of forensic sciences (2021): 700-708