MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] CAS 6160-78-7

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Physical properties

Molecular weight	338.32
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

6160-78-7

Molecular weight

338.32

Excitation (nm)

341

Emission (nm)

441

4-Methylumbelliferyl-beta-D-galactopyranoside (MUG) is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	295.578 µL	1.478 mL	2.956 mL	14.779 mL	29.558 mL
5 mM	59.116 µL	295.578 µL	591.156 µL	2.956 mL	5.912 mL
10 mM	29.558 µL	147.789 µL	295.578 µL	1.478 mL	2.956 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	341
Emission (nm)	441

Images

Figure 1. Chemical structure for MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7*

References

View all 18 references: Citation Explorer

Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice
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Journal: Cancer Gene Ther (2006): 367

Characterization of an unusual cold-active beta-glucosidase belonging to family 3 of the glycoside hydrolases from the psychrophilic isolate Paenibacillus sp. strain C7
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Journal: Appl Environ Microbiol (2005): 4225

Catheter-based antegrade intracoronary viral gene delivery with coronary venous blockade
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Journal: Am J Physiol Heart Circ Physiol (2005): H2995

Neuronal Nitric Oxide Synthase Activity in the Paraventricular Nucleus Buffers Central Endothelin-1- induced Pressor Response and Vasopressin Secretion
Authors: Rossi NF, Black SM, Telemaque-Potts S, Chen H.
Journal: J Cardiovasc Pharmacol (2004): S283

A novel synthetic peptide derivative from lactoferrin exhibiting antimicrobial activity
Authors: Ramesh CV, Kamini NR, Puvanakrishnan R.
Journal: Biotechnol Appl Biochem (2004): 271

Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans
Authors: Honeyman AL, Cote CK, Curtiss R, 3rd.
Journal: J Microbiol Methods (2002): 163

Optimisation of an enzymatic method for beta-galactosidase
Authors: McGuire JB, James TJ, Imber CJ, St Peter SD, Friend PJ, Taylor RP.
Journal: Clin Chim Acta (2002): 123

Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases
Authors: Gee KR, Sun WC, Bhalgat MK, Upson RH, Klaubert DH, Latham KA, Haugl and RP., undefined
Journal: Anal Biochem (1999): 41

Adenoviral gene transfer to spinal-cord neurons: intrathecal vs. intraparenchymal administration
Authors: Mannes AJ, Caudle RM, O'Connell BC, Iadarola MJ.
Journal: Brain Res (1998): 1

Comparative performance of Colisure
Authors: McFeters GA, Broadaway SC, Pyle BH, Pickett M, Egozy Y.
Journal: J Am Water Works Assoc (1997): 112