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MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7*

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Physical properties
Molecular weight338.32
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
Excitation (nm)
Emission (nm)
4-Methylumbelliferyl-beta-D-galactopyranoside (MUG) is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MUG [4-Methylumbelliferyl-beta-D-galactopyranoside] *CAS 6160-78-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM295.578 µL1.478 mL2.956 mL14.779 mL29.558 mL
5 mM59.116 µL295.578 µL591.156 µL2.956 mL5.912 mL
10 mM29.558 µL147.789 µL295.578 µL1.478 mL2.956 mL

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Spectral properties

Excitation (nm)341
Emission (nm)441



View all 18 references: Citation Explorer
Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice
Authors: Louis MH, Dutoit S, Denoux Y, Erbacher P, Desl and es E, Behr JP, Gauduchon P, Poulain L.
Journal: Cancer Gene Ther (2006): 367
Characterization of an unusual cold-active beta-glucosidase belonging to family 3 of the glycoside hydrolases from the psychrophilic isolate Paenibacillus sp. strain C7
Authors: Shipkowski S, Brenchley JE.
Journal: Appl Environ Microbiol (2005): 4225
Catheter-based antegrade intracoronary viral gene delivery with coronary venous blockade
Authors: Hayase M, Del Monte F, Kawase Y, Macneill BD, McGregor J, Yoneyama R, Hoshino K, Tsuji T, De Gr and AM, Gwathmey JK, Frangioni JV, Hajjar RJ.
Journal: Am J Physiol Heart Circ Physiol (2005): H2995
Neuronal Nitric Oxide Synthase Activity in the Paraventricular Nucleus Buffers Central Endothelin-1- induced Pressor Response and Vasopressin Secretion
Authors: Rossi NF, Black SM, Telemaque-Potts S, Chen H.
Journal: J Cardiovasc Pharmacol (2004): S283
A novel synthetic peptide derivative from lactoferrin exhibiting antimicrobial activity
Authors: Ramesh CV, Kamini NR, Puvanakrishnan R.
Journal: Biotechnol Appl Biochem (2004): 271
Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans
Authors: Honeyman AL, Cote CK, Curtiss R, 3rd.
Journal: J Microbiol Methods (2002): 163
Optimisation of an enzymatic method for beta-galactosidase
Authors: McGuire JB, James TJ, Imber CJ, St Peter SD, Friend PJ, Taylor RP.
Journal: Clin Chim Acta (2002): 123
Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases
Authors: Gee KR, Sun WC, Bhalgat MK, Upson RH, Klaubert DH, Latham KA, Haugl and RP., undefined
Journal: Anal Biochem (1999): 41
Adenoviral gene transfer to spinal-cord neurons: intrathecal vs. intraparenchymal administration
Authors: Mannes AJ, Caudle RM, O'Connell BC, Iadarola MJ.
Journal: Brain Res (1998): 1
Comparative performance of Colisure
Authors: McFeters GA, Broadaway SC, Pyle BH, Pickett M, Egozy Y.
Journal: J Am Water Works Assoc (1997): 112