MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

MUP

4-Methylumbelliferyl phosphate, free acid; CAS 3368-04-5

A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.

Protocol

COA

Catalog	Size	Price	Quantity
11614	25 mg	Price
11617	5 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	390.396 µL	1.952 mL	3.904 mL	19.52 mL	39.04 mL
5 mM	78.079 µL	390.396 µL	780.793 µL	3.904 mL	7.808 mL
10 mM	39.04 µL	195.198 µL	390.396 µL	1.952 mL	3.904 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	256.15 g/mol	=	mL	x	mM	=	mmol

References

View all 76 references: Citation Explorer

Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro

Authors:

Bednarska K, Klink M, Sulowska Z.

Journal:

Mediators Inflamm (2006): 19307

Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages

Authors:

Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.

Journal:

Lett Appl Microbiol (2006): 438

Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition

Authors:

Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.

Journal:

J Virol Methods (2005): 33

Relative movement and soil fixation of soluble organic and inorganic phosphorus

Authors:

Anderson BH, Magdoff FR.

Journal:

J Environ Qual (2005): 2228

Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides

Authors:

Hoehamer CF, Mazur CS, Wolfe NL.

Journal:

J Agric Food Chem (2005): 90

Physical properties

Molecular weight	256.15
Solvent	Water

Spectral properties

Correction factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	3368-04-5

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Page updated on October 9, 2025