MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] CAS 22919-26-2

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Physical properties

Molecular weight	300.11
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

22919-26-2

Molecular weight

300.11

Correction Factor (280 nm)

0.1

Extinction coefficient (cm ^-1 M ^-1)

18000

Excitation (nm)

360

Emission (nm)

448

A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	333.211 µL	1.666 mL	3.332 mL	16.661 mL	33.321 mL
5 mM	66.642 µL	333.211 µL	666.422 µL	3.332 mL	6.664 mL
10 mM	33.321 µL	166.606 µL	333.211 µL	1.666 mL	3.332 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein, disodium salt CAS 518-47-8	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

References

View all 76 references: Citation Explorer

Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307

Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
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Journal: Lett Appl Microbiol (2006): 438

Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
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Journal: J Virol Methods (2005): 33

Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228

Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90

Crystal structure of a covalent intermediate of endogenous human arylsulfatase A
Authors: Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K.
Journal: J Inorg Biochem (2003): 386

Enzymatic differentiation of Candida parapsilosis from other Candida spp. in a membrane filtration test
Authors: Bauters TG, Peleman R, Dhont M, Vanhaesebrouck P, Nelis HJ.
Journal: J Microbiol Methods (2003): 11

Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species
Authors: Shroyer ML, Bhunia AK.
Journal: J Microbiol Methods (2003): 35

LVV-hemorphin-4 modulates Ca2+/calmodulin-dependent pathways in the immune system by the same mechanism as in the brain
Authors: Barkhudaryan N, Gambarov S, Gyulbayazyan T, Nahapetyan K.
Journal: J Mol Neurosci (2002): 203

A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration
Authors: Bouaicha N, Maatouk I, Vincent G, Levi Y.
Journal: Food Chem Toxicol (2002): 1677