MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
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Physical properties
Molecular weight | 300.11 |
Solvent | Water |
Spectral properties
Correction Factor (280 nm) | 0.1 |
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 360 |
Emission (nm) | 448 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Alkaline Phosphatase (ALP), Biotin and Streptavidin, Coumarins, Enzyme-Linked Immunosorbent Assay (ELISA), Secondary Antibodies
CAS 22919-26-2 | Molecular weight 300.11 | Correction Factor (280 nm) 0.1 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 360 | Emission (nm) 448 |
A fluorogenic substrate for phosphatases. It is widely used for detecting phosphatases in solution. However this phosphatase substrate is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer MUP Plus that is developed to address this pH limitation associated with MUP substrates.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 333.211 µL | 1.666 mL | 3.332 mL | 16.661 mL | 33.321 mL |
5 mM | 66.642 µL | 333.211 µL | 666.422 µL | 3.332 mL | 6.664 mL |
10 mM | 33.321 µL | 166.606 µL | 333.211 µL | 1.666 mL | 3.332 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.1 |
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 360 |
Emission (nm) | 448 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluorescein, disodium salt *CAS 518-47-8* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
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References
View all 76 references: Citation Explorer
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Crystal structure of a covalent intermediate of endogenous human arylsulfatase A
Authors: Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K.
Journal: J Inorg Biochem (2003): 386
Authors: Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K.
Journal: J Inorg Biochem (2003): 386
Enzymatic differentiation of Candida parapsilosis from other Candida spp. in a membrane filtration test
Authors: Bauters TG, Peleman R, Dhont M, Vanhaesebrouck P, Nelis HJ.
Journal: J Microbiol Methods (2003): 11
Authors: Bauters TG, Peleman R, Dhont M, Vanhaesebrouck P, Nelis HJ.
Journal: J Microbiol Methods (2003): 11
Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species
Authors: Shroyer ML, Bhunia AK.
Journal: J Microbiol Methods (2003): 35
Authors: Shroyer ML, Bhunia AK.
Journal: J Microbiol Methods (2003): 35
LVV-hemorphin-4 modulates Ca2+/calmodulin-dependent pathways in the immune system by the same mechanism as in the brain
Authors: Barkhudaryan N, Gambarov S, Gyulbayazyan T, Nahapetyan K.
Journal: J Mol Neurosci (2002): 203
Authors: Barkhudaryan N, Gambarov S, Gyulbayazyan T, Nahapetyan K.
Journal: J Mol Neurosci (2002): 203
A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration
Authors: Bouaicha N, Maatouk I, Vincent G, Levi Y.
Journal: Food Chem Toxicol (2002): 1677
Authors: Bouaicha N, Maatouk I, Vincent G, Levi Y.
Journal: Food Chem Toxicol (2002): 1677
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets