Name: MycoLight™ Cyclic-di-GMP Red Sensor
Brand: AAT Bioquest
SKU: 17103
Price: 495 USD
Availability: InStock

MycoLight™ Cyclic-di-GMP Red Sensor

MycoLight™ Cyclic-di-GMP Red Sensor is a selective fluorescent probe that binds bacterial second messenger c-di-GMP with red emission, enabling detection of biofilm formation and bacterial virulence regulation.

Selective c-di-GMP Binding: Specifically detects bacterial second messenger with minimal interference from cAMP or cGMP
Red Fluorescence Emission: Long wavelength detection minimizes autofluorescence in bacterial samples
Biofilm Formation Monitoring: Tracks c-di-GMP levels associated with bacterial virulence and antibiotic resistance
Live-Cell Compatible: Enables real-time detection of bacterial signaling in physiological conditions

The fluorescence intensity of MycoLight™ Cyclic-di-GMP Red Sensor was measured at 650 nm (excitation at 490 nm) in the presence of Cyclic-di-GMP (Blue), dNTPs (Red) or cyclic-AMP (Green) ranging from 400 to 6.125 µM in 10 mM Tris-HCl, pH 7.5 containing 60 mM KCl buffer. MycoLight™ Cyclic-di-GMP Red Sensor is specific to Cyclic-di-GMP and has no response for dNTPs and cyclic-AMP.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17103	1 mg	Price

Overview

The MycoLight™ Cyclic-di-GMP Red Sensor is developed to fluorometrically detect cyclic-di-GMP (c-di-GMP). The sensor generates a red fluorescent signal upon selectively binding to c-di-GMP. The second messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP) is ubiquitous in bacteria and regulates a wide range of bacterial functions, including biofilm formation, which can lead to chronic infections and antibiotic resistance. C-di-GMP exists in both monomer and dimer forms. Currently, there is a lack of sensitive probes for detecting c-di-GMP. The MycoLight™ Cyclic-di-GMP Red Sensor addresses this unmet need, offering high sensitivity and selectivity towards the c-di-GMP structure with minimal interference from other secondary messengers like cyclic AMP. Its larger Stokes shift provides improved signals and allows for multiplexing with other fluorophores in imaging applications without spectral overlap.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MycoLight™ Cyclic-di-GMP Red Sensor to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	210.301 µL	1.052 mL	2.103 mL	10.515 mL	21.03 mL
5 mM	42.06 µL	210.301 µL	420.601 µL	2.103 mL	4.206 mL
10 mM	21.03 µL	105.15 µL	210.301 µL	1.052 mL	2.103 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	475.51 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Harnessing Cyclic di-GMP Signaling: A Strategic Approach to Combat Bacterial Biofilm-Associated Chronic Infections.

Authors:

Snega Priya, P and Meenatchi, Ramu and Pasupuleti, Mukesh and Namasivayam, S Karthick Raja and Arockiaraj, Jesu

Journal:

Current microbiology (2025): 118

Cyclic di-GMP inhibits nitrate assimilation by impairing the antitermination function of NasT in Pseudomonas putida.

Authors:

Nie, Liang and Xiao, Yujie and Zhou, Tiantian and Feng, Haoqi and He, Meina and Liang, Qingyuan and Mu, Kexin and Nie, Hailing and Huang, Qiaoyun and Chen, Wenli

Journal:

Nucleic acids research (2024): 186-203

Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms.

Authors:

Liao, Hebin and Yan, Xiaodan and Wang, Chenyi and Huang, Chun and Zhang, Wei and Xiao, Leyi and Jiang, Jun and Bao, Yongjia and Huang, Tao and Zhang, Hanbo and Guo, Chunming and Zhang, Yufeng and Pu, Yingying

Journal:

eLife (2024)

Negative feedback of cyclic di-GMP levels optimizes switching between sessile and motile lifestyles in Vibrio cholerae.

Authors:

Rangarajan, Aathmaja Anandhi and Schroeder, Jeremy W and Hurto, Rebecca L and Severin, Geoffrey B and Pell, Macy E and Hsieh, Meng-Lun and Waters, Christopher M and Freddolino, Lydia

Journal:

bioRxiv : the preprint server for biology (2024)

A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.

Authors:

Shi, Yu and Cheng, Tianfang and Cheang, Qing Wei and Zhao, Xiaoyan and Xu, Zeling and Liang, Zhao-Xun and Xu, Linghui and Wang, Junxia

Journal:

Molecular plant pathology (2024): e13496

Physical properties

Molecular weight	475.51
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 8, 2025