MycoLight™ Flow Cytometric Live Bacteria Assay Kit

The MycoLight Flow Cytometric Live Bacteria Assay Kit provides an easy and convenient methodfor evaluating bacterial vitality as a function of the intracellular esterase activity. MycoLight™ 520 is non-fluorescent esterase substrate that diffuse into both Gram positive and Gram-negative bacteria. Upon hydrolysis by bacterial intracellular non-specific esterase, a green fluorescent product is produced and accumulated within bacteria. Compared to the commonly used esterase substrate CFDA and CFDA-AM, this kit provides brighter and more stable signal with lower background and easier staining protocol.

Relative viability of <em>E.coli</em> suspension was analyzed using ACEA NovoCyte flow cytometer in FITC channel. The readings (Count(%)) were obtained from various Live/Dead <em>E.coli</em> mixtures (Table 1). The live and dead population in each mixture can be easily distinguished by the two distinct peaks.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22407	100 Tests	Price

Citations

View all 13 citations: Citation Explorer

Seminal plasma affects the survival rate and motility pattern of raw llama spermatozoa

Authors:

Fumuso, F. G., Giuliano, S. M., Chaves, M. G., Neild, D. M., Miragaya, M. H., Gambarotta, M. C., Carretero, M. I.

Journal:

Anim Reprod Sci (2018): 99-106

New fluorescein precursors for live bacteria detection

Authors:

Guilini, C., Baehr, C., Schaeffer, E., Gizzi, P., Rufi, F., Haiech, J., Weiss, E., Bonnet, D., Galzi, J. L.

Journal:

Anal Chem (2015): 8858-66

Protective effects of antithrombin on free groin flaps after secondary venous stasis in the rat model

Authors:

Wallmichrath, J., Knab, R., Baumeister, R. G., Volkmer, E., Giunta, R. E., Frick, A.

Journal:

J Plast Reconstr Aesthet Surg (2014): 707-11

Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm

Authors:

Tawakoli, P. N., Al-Ahmad, A., Hoth-Hannig, W., Hannig, M., Hannig, C.

Journal:

Clin Oral Investig (2013): 841-50

Rapid flow cytometric method for viability determination of solventogenic clostridia

Authors:

Linhova, M., Branska, B., Patakova, P., Lipovsky, J., Fribert, P., Rychtera, M., Melzoch, K.

Journal:

Folia Microbiol (Praha) (2012): 307-11

Spectral properties

Correction factor (260 nm)	0.31
Correction factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

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Page updated on October 13, 2025

Component A: MycoStain It™ 520	2 vials
Component B: Assay Buffer	1 bottle (50 mL)
Component C: DMSO	1 vial (200 µL)