MycoLight™ Fluorimetric CTC Live Bacteria Quantification Kit

The CTC Flow Cytometric Live Bacteria Assay Kit provides an easy and convenient method for evaluating bacterial health and vitality as a function of the respiratory activity. CTC itself is non-fluorescent, once reduced by the electron transfer system of viable bacterial cell surface, red and insoluble fluorescent CTC formazan is formed and can be detected with a flow cytometer. Dead bacteria that are not respiring or unhealthy bacteria that respire at a lower rate will produce none or less red fluorescent with CTC staining, thus providing a semi-quantitative measure of health status of bacteria population.

Live and dead (ethanol treated and boiled) <em>E.coli</em> were stained with 1X of CTC for 30 mins at 37°C. Samples were analyzed by flow cytometer with a 488 nm excitation and 615/24 nm bandpass filter. Live and dead bacteria population showed very distinct peaks.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22405	100 Tests	Price

Citations

View all 25 citations: Citation Explorer

Assessing the effectiveness and mechanisms of Fe/PS, Fe/H2O2, and O3 treatment for water disinfection: Spotlight on VBNC bacteria

Authors:

Qi, Zheng and Huang, Zaihui and Liu, Chunguang

Journal:

Journal of Environmental Sciences (2024)

Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method that Minimizes ROS Measurement Artifacts with Fluorescent Dyes

Authors:

McBee, M. E., Chionh, Y. H., Sharaf, M. L., Ho, P., Cai, M. W., Dedon, P. C.

Journal:

Front Microbiol (2017): 459

Effect of extremely low frequency electromagnetic fields on bacterial membrane

Authors:

Oncul, S., Cuce, E. M., Aksu, B., Inhan Garip, A.

Journal:

Int J Radiat Biol (2016): 42-9

Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection

Authors:

Hu, W., Zhang, Y., Yang, H., Yu, J., Wei, H.

Journal:

J Microbiol Methods (2015): 47-53

Reduction of biofilm formation on titanium surface with ultraviolet-C pre-irradiation

Authors:

Yamada, Y., Yamada, M., Ueda, T., Sakurai, K.

Journal:

J Biomater Appl (2014): 161-171

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	610/20 nm filter
Instrument specification(s)	PE-Texas Red channel

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Page updated on September 25, 2025

Component A: CTC	1 vial
Component B: Assay Buffer	1 bottle (100 mL)