MycoLight™ Green JJ99 *5 mM in DMSO*

MycoLight™ Green JJ99

5 mM in DMSO

MycoLight™ Green JJ99 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes. MycoLight™ Green JJ99 stain has a high affinity for DNA and exhibits enhanced fluorescence upon binding with an excitation maximum close to the 488 nm argon laser line and fluorescence emission maximum at ≤500 nm. MycoLight™ Green JJ99 stain is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead Gram-positive and Gram-negative bacteria. It is an excellent replacement for SYTO^® 9 (SYTO^® is the trademark of Invitrogen).

<em>E.Coli</em> were stained with 5 uM of MycoLight™ Green JJ99 for 30 minutes and imaged with FITC channel.

Protocol

SDS

Catalog	Size	Price	Quantity
24001	100 ul	Price

References

View all 25 references: Citation Explorer

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye

Authors:

Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.

Journal:

Am J Trop Med Hyg (2014): 306

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9

Authors:

Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.

Journal:

Clin Lab (2013): 795

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death

Authors:

Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.

Journal:

BMC Res Notes (2012): 437

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye

Authors:

Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.

Journal:

Mol Cell Probes (2012): 121

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes

Authors:

Wlodkowic D, Skommer J, Darzynkiewicz Z.

Journal:

Methods Mol Biol (2011): 81

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	482
Emission (nm)	512

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Nucleic Acid Detection Probes & Assay Kits

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Page updated on October 31, 2025