MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit

The MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit provides an easy and convenient way for determination of gram sign in live bacteria. Gram staining is a commonly used method in both clinical and research settings to taxonomically classify bacterial species into two large groups. Unfortunately, the traditional gram staining method is tedious and involves bacterial fixation which can be a significant drawback if the bacteria are to be characterized further. The MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit provides a one-step gram staining assay for live bacteria that overcomes the problems inherent in the traditional gram staining assays. The MycoLight™ Rapid Fluorescence Bacterial Gram Stain Kit utilizes two DNA dyes MycoLight™ Green and MycoLight™ Red with differential ability to stain gram positive and negative bacteria. MycoLight™ Green stains both gram positive and negative bacteria while MycoLight™ Red preferentially labels gram positive bacteria. The excitation/emission maxima for these two dyes are about 484/504 nm for MycoLight™ Green and 650/669 nm for MycoLight™ Red. Thus, when a mixture of gram positive and gram negative bacteria is stained with the dyes, gram positive bacteria will fluoresce red and gram negative bacteria will fluoresce green. The gram positive and negative staining can be monitored fluorimeterically with Cy5 and FITC filter set respectively.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22413	100 Tests	Price

Citations

View all 45 citations: Citation Explorer

In vitro efficacy of Er: YAG laser-activated irrigation versus passive ultrasonic irrigation and sonic-powered irrigation for treating multispecies biofilms in artificial grooves and dentinal tubules: an SEM and CLSM study

Authors:

Bao, Pingping and Liu, He and Yang, Lan and Zhang, Lulu and Yang, Liwei and Xiao, Nannan and Shen, Jing and Deng, Jiayin and Shen, Ya

Journal:

BMC Oral Health (2024): 1--14

TiO2-DNA Nanosensor In Situ for Quick Detection of Nasal Flora in Allergic Rhinitis Patients

Authors:

Chen, Weihua and Zhang, Kaiyang and Zhong, Zewei

Journal:

Computational and Mathematical Methods in Medicine (2022)

Gram Stain and Molecular Method for the Diagnosis of Bacterial Pneumonia

Authors:

Guo, X. G., Liu, Q. F.

Journal:

Chin Med J (Engl) (2016): 1884

Combination of Gram Stain, Sputum Culture, and Molecular Method for Diagnosis and Guiding Target Therapies of Bacterial Pneumonia

Authors:

Wang, F., Gao, Z. C.

Journal:

Chin Med J (Engl) (2016): 1885

Diagnosis of common bacterial causes of urethritis in men by Gram stain, culture and multiplex PCR

Authors:

Jahan, F., Shamsuzzaman, S. M., Akter, S.

Journal:

Malays J Pathol (2014): 175-80

Spectral properties

Excitation (nm)	482
Emission (nm)	512

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	488/650 nm
Emission	530/669 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC/Cy5 filters

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 23, 2025

Component A: MycoLight™ Green	1 vial (100 µL)
Component B: MycoLight™ Red	1 vial