MycoLight™ Ratiometric Bacterial Membrane Potential Kit *Red/Green Fluorescence*

MycoLight™ Ratiometric Bacterial Membrane Potential Kit

Red/Green Fluorescence

AAT Bioquest's MycoLight™ Ratiometric Bacterial Membrane Potential Kit uses a fluorescent sensor that exhibits green fluorescence in both gram-positive and negative bacterial cells in low concentration, but the fluorescence shifts toward red emission at higher cytosolic concentrations due to the dye molecule aggregation caused by larger membrane potentials. The magnitude of membrane potential varies with different bacterial species. For many gram-positive species, the red/green ratio tend to vary with the intensity of the proton gradient while in many gram-negative bacteria the response of the dye does not appear to be proportional to proton gradient intensity. This kit is designed to assay bacterial membrane potentials when the bacterial concentrations are in the range of 105 “ 107 organisms per mL. Stained cells can be monitored fluorimetrically at 510-530 nm (FITC filter) and 600-660 nm (Texas red filter) with excitation at 488 nm, the most common excitation light source.

<p><em>Bacillus </em><em>subtilis </em>was cultured to log phase and diluted to the concentration of 1 x 10<sup>6</sup> cells per mL in PBS. Cells were then treated with 5 µM CCCP for 20 min and incubated with 1X MycoStain It™ Green for 30 min before flow cytometry analysis.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22401	200 Tests	Price

References

View all 16 references: Citation Explorer

Raman spectroscopic analysis of Lactobacillus rhamnosus GG in response to dehydration reveals DNA conformation changes

Authors:

Myintzu Hlaing, M.; Wood, B.; McNaughton, D.; Ying, D.; Augustin, M. A.

Journal:

J Biophotonics (2017): 589-597

Antibacterial and antigelatinolytic effects of Satureja hortensis L. essential oil on epithelial cells exposed to Fusobacterium nucleatum

Authors:

Zeidan-Chulia, F.; Keskin, M.; Kononen, E.; Uitto, V. J.; Soderling, E.; Moreira, J. C.; Gursoy, U. K.

Journal:

J Med Food (2015): 503-6

Inactivation of Cronobacter sakazakii in reconstituted infant formula by combination of thymoquinone and mild heat

Authors:

Shi, C.; Jia, Z.; Chen, Y.; Yang, M.; Liu, X.; Sun, Y.; Zheng, Z.; Zhang, X.; Song, K.; Cui, L.; Baloch, A. B.; Xia, X.

Journal:

J Appl Microbiol (2015): 1700-6

Fourier transform infra-red spectroscopy and flow cytometric assessment of the antibacterial mechanism of action of aqueous extract of garlic (Allium sativum) against selected probiotic Bifidobacterium strains

Authors:

Booyens, J.; Thantsha, M. S.

Journal:

BMC Complement Altern Med (2014): 289

Observation of injured E. coli population resulting from the application of high-pressure throttling treatments

Authors:

De Lamo-Castellvi, S.; Toledo, R.; Frank, J. F.

Journal:

J Food Sci (2013): M582-6

Spectral properties

Excitation (nm)	483
Emission (nm)	501

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm, 610/20 nm filter
Instrument specification(s)	FITC, PE-Texas Red channel

Fluorescence microscope
Excitation	510/600 nm
Emission	530/660 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC/Texas Red filter sets

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Page updated on October 8, 2025

Component A: MycoStain It™ Green	1 vial (1 mL-100X)
Component B: CCCP	1 vial (300 µL-500 µM in DMSO)
Component C: PBS	100 mL