logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
MycoLight™ Red JJ94
2.5 mM in DMSO
Green fluorescent protein (GFP) derivatives are widely used as fluorescent reporters to study biological processes at the cellular and molecular level, including labeling bacteria. Unfortunately, the GFP-based bacterial labeling is tedious, and several important organisms have remained difficult to modify for fluorescent protein expression. Complimentary to GFP-based fluorescence labeling of bacteria, SYTO-9 has been widely used for labeling bacteria in a more convenient mode, i.e., through a simple incubation. However, SYTO-9 is reported to significantly inhibit some bacterial growth. AAT Bioquest has developed MycoLight™ Red JJ94 that has minimal inhibition of bacterial growth compared to SYTO-9. In addition, MycoLight™ Red JJ94 can be used for multicolor detection of bacteria with the widely used GFP probes. It has an excitation and emission at ~637 and ~651 nm, respectively. This perfectly matches the He-laser excitation and Cy5 filter set that are commonly equipped in most fluorescence microscopes and flow cytometers. MycoLight™ Red JJ94 is a far-red fluorescent DNA-binding dye for straightforward live bacterial cell labeling, allowing subsequent infection studies. It has low intrinsic fluorescence, but upon binding DNA, its fluorescence emission intensity increases dramatically. MycoLight™ Red JJ94 readily stains live bacteria without compromising microbial growth. Under the same conditions, SYTO-9 inhibits bacterial growth substantially while MycoLight™ Red JJ94 is fully compatible with normal bacterial growth in liquid medium or on solid medium. The bacterial fluorescence labeling by MycoLight™ Red JJ94 is stable over several hours after shifting the bacteria into dye-free media.
Optical density (OD600 nm) of <em>E. coli</em> LB cultures supplemented with 1% DMSO (control), MycoLight<sup>TM</sup>&nbsp; Red JJ94 or Syto-9<sup>TM</sup> &nbsp;over time. Syto-9<sup>TM</sup> inhibits bacterial growth substantially while MycoLight&trade; Red JJ94 is fully compatible with normal bacterial growth.
Optical density (OD600 nm) of <em>E. coli</em> LB cultures supplemented with 1% DMSO (control), MycoLight<sup>TM</sup>&nbsp; Red JJ94 or Syto-9<sup>TM</sup> &nbsp;over time. Syto-9<sup>TM</sup> inhibits bacterial growth substantially while MycoLight&trade; Red JJ94 is fully compatible with normal bacterial growth.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
24006100 uL
Price
 
Physical properties
Molecular weight738.75
SolventDMSO
Storage, safety and handling
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
Excitation630 nm
Emission660 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy5 filterset
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 9, 2025