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N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin [N-Methoxysuccinyl-AAPV-AMC]

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin has been used as a fluorogenic substrate in determining the elastase activity from bone marrow lysates and pancreatic lysates. It is also used for detecting the neutrophil elastase in bronchial epithelial cells. The protease activity is measured by fluorescence spectrophotometry with excitation wavelength at ~370 nm and emission wavelength at ~460 nm.

Spectrum

References

View all 50 references: Citation Explorer
Clinical utility of the neutrophil elastase inhibitor sivelestat for the treatment of ALI/ARDS patients with COVID-19.
Authors: Wang, Ruiying and Yin, Junping and Li, Jian and Bai, Xueli and Liu, Hu and Cheng, Mengyu and Wang, Lei and Chen, Yuan and Wei, Shuang and Liu, Xiansheng
Journal: Heliyon (2024): e36337
[Sivelestat sodium inhibits neutrophil elastase to regulate intrahepatic biliary mucin 5AC expression].
Authors: Gu, Ximao and Ye, Haijun and Xu, Chenglei and Lin, Zhuying and Li, Jiang
Journal: Zhonghua wei zhong bing ji jiu yi xue (2024): 609-615
The incidence rate and histological characteristics of intimal hyperplasia in elastase-induced experimental abdominal aortic aneurysms in mice.
Authors: Li, Meng and Wei, Panpan and Li, Kexin and Liu, Haole and Alam, Naqash and Hou, Haiwen and Deng, Jie and Xu, Baohui and Liu, Enqi and Zhao, Sihai and Li, Yankui
Journal: Animal models and experimental medicine (2024): 388-395
PD-L1 siRNA hitched polyethyleneimine-elastase constituting nanovesicle induces tumor immunogenicity and PD-L1 silencing for synergistic antitumor immunotherapy.
Authors: Du, Li and Gong, Yao and Zhang, Xiaoying and Sun, Jide and Gao, Fengxia and Shen, Meiying and Bai, Huili and Yang, Tiantian and Cheng, Xiaoxue and Li, Siqiao and Peng, Jian and Liu, Zhangling and Ding, Shijia and Chen, Junman and Cheng, Wei
Journal: Journal of nanobiotechnology (2024): 442
Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral ß-aminopropionitrile.
Authors: Bontekoe, Jack and Upchurch, Gilbert and Morgan, Courtney and Liu, Bo
Journal: Journal of visualized experiments : JoVE (2024)
Page updated on May 19, 2025

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Catalog Number13419
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Physical properties

Molecular weight

627.70

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

CAS

72252-90-5
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