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NAADP-AM
NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger. NAADP is negatively charged (to prevent it from leaking out of cells). The negative charge of NAADP makes it well retained in cells, but imposes difficulties in loading NAADP into live cells. NAADP must be injected, loaded via liposomes, or electroporated. These invasive techniques are highly technically demanding and are possible only in certain single cell preparations. NAADP-AM has been reported as a cell-permeant analog of NAADP. NAADP-AM can be taken into cells and induces NAADP-mediated calcium signaling. NAADP-AM can be used in a wide range of systems and will greatly facilitate research into the role of NAADP in biological research. The NAADP-AM consists of three NAADP AM esters with one major component and two minor AM esters.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of NAADP-AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 96.839 µL | 484.196 µL | 968.392 µL | 4.842 mL | 9.684 mL |
| 5 mM | 19.368 µL | 96.839 µL | 193.678 µL | 968.392 µL | 1.937 mL |
| 10 mM | 9.684 µL | 48.42 µL | 96.839 µL | 484.196 µL | 968.392 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 7 citations: Citation Explorer
Lysosomal TPC2 channel as a new target of chlorpromazine and Clomipramine to induce protective autophagy in L-BMAA-induced neurodegeneration
Authors: Tedeschi, Valentina and Ciancio, Raffaella and Magliocca, Giorgia and Esposito, Emilia and Piccirillo, Silvia and Rubino, Valentina and Preziuso, Alessandra and Spadoni, Tatiana and Di Muraglia, Noemi and Ruggiero, Giuseppina and others,
Journal: Biochemical Pharmacology (2025): 117219
Authors: Tedeschi, Valentina and Ciancio, Raffaella and Magliocca, Giorgia and Esposito, Emilia and Piccirillo, Silvia and Rubino, Valentina and Preziuso, Alessandra and Spadoni, Tatiana and Di Muraglia, Noemi and Ruggiero, Giuseppina and others,
Journal: Biochemical Pharmacology (2025): 117219
MASTER-NAADP: a membrane permeable precursor of the Ca2+ mobilizing second messenger NAADP
Authors: Krukenberg, Sarah and M{\"o}ckl, Franziska and Wei{\ss}, Mariella and Dekiert, Patrick and Hofmann, Melanie and Gerlach, Fynn and Winterberg, Kai J and Kovacevic, Dejan and Khansahib, Imrankhan and Troost, Berit and others,
Journal: Nature Communications (2024): 8008
Authors: Krukenberg, Sarah and M{\"o}ckl, Franziska and Wei{\ss}, Mariella and Dekiert, Patrick and Hofmann, Melanie and Gerlach, Fynn and Winterberg, Kai J and Kovacevic, Dejan and Khansahib, Imrankhan and Troost, Berit and others,
Journal: Nature Communications (2024): 8008
Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)-Mediated Calcium Signaling Is Active in Memory CD4+ T Cells
Authors: Chakraborty, Anish and Dissanayake, Ravindika and Wall, Katherine A
Journal: Molecules (2024): 907
Authors: Chakraborty, Anish and Dissanayake, Ravindika and Wall, Katherine A
Journal: Molecules (2024): 907
LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells
Authors: Nabar, Neel R and Heijjer, Christopher N and Shi, Chong-Shan and Hwang, Il-Young and Ganesan, Sundar and Karlsson, Mikael CI and Kehrl, John H
Journal: Autophagy (2021): 1--19
Authors: Nabar, Neel R and Heijjer, Christopher N and Shi, Chong-Shan and Hwang, Il-Young and Ganesan, Sundar and Karlsson, Mikael CI and Kehrl, John H
Journal: Autophagy (2021): 1--19
NAADP-induced intracellular calcium ion is mediated by the TPCs (two-pore channels) in hypoxia-induced pulmonary arterial hypertension
Authors: Hu, Wen and Zhao, Fei and Chen, Ling and Ni, Jiamin and Jiang, Yongliang
Journal: Journal of Cellular and Molecular Medicine (2021)
Authors: Hu, Wen and Zhao, Fei and Chen, Ling and Ni, Jiamin and Jiang, Yongliang
Journal: Journal of Cellular and Molecular Medicine (2021)
References
View all 149 references: Citation Explorer
Both RyRs and TPCs are required for NAADP-induced intracellular Ca release
Authors: Gerasimenko JV, Charlesworth RM, Sherwood MW, Ferdek PE, Mikoshiba K, Parrington J, Petersen OH, Gerasimenko OV.
Journal: Cell Calcium. (2015)
Authors: Gerasimenko JV, Charlesworth RM, Sherwood MW, Ferdek PE, Mikoshiba K, Parrington J, Petersen OH, Gerasimenko OV.
Journal: Cell Calcium. (2015)
TPC: the NAADP discovery channel
Authors: Morgan AJ, Davis LC, Ruas M, Galione A.
Journal: Biochem Soc Trans (2015): 384
Authors: Morgan AJ, Davis LC, Ruas M, Galione A.
Journal: Biochem Soc Trans (2015): 384
Role of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in Keratinocyte Differentiation
Authors: Park KH, Kim KN, Park DR, Jang KY, Kim UH.
Journal: J Invest Dermatol (2015): 1692
Authors: Park KH, Kim KN, Park DR, Jang KY, Kim UH.
Journal: J Invest Dermatol (2015): 1692
A primer of NAADP-mediated Ca signalling: From sea urchin eggs to mammalian cells
Authors: Galione A., undefined
Journal: Cell Calcium (2015): 27
Authors: Galione A., undefined
Journal: Cell Calcium (2015): 27
The role of calcium and nicotinic acid adenine dinucleotide phosphate (NAADP) in human osteoclast formation and resorption
Authors: Cheng X, Hookway ES, Kashima T, Oppermann U, Galione A, Athanasou NA.
Journal: Calcif Tissue Int (2015): 73
Authors: Cheng X, Hookway ES, Kashima T, Oppermann U, Galione A, Athanasou NA.
Journal: Calcif Tissue Int (2015): 73
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