Name: NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*
Brand: AAT Bioquest
SKU: 825
Price: 90 USD
Availability: InStock

NBD-Cl

4-Chloro-7-nitrobenzofurazan; CAS 10199-89-0

NBD-Cl is widely used to label peptides, proteins, drugs and other biomolecules. It is also a popular derivatizing reagent for HPLC analysis. NBD-Cl is non-fluorescent, and generates fluorescent adducts upon reacting with aliphatic amines or thiol compounds. It reacts with amino groups such as aliphatic amines, amino acids, peptides, and proteins to form highly fluorescent compounds. The NBD-amine adducts have ?ex = 464 nm and ?em = 512 nm in aqueous solutions. The fluorescence spectra of NBD-amine adducts are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions. NBD-Cl also reacts with thiol group to form fluorescent adducts, but the thiol adducts are much less fluorescent than the amine adducts.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
825	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	501.128 µL	2.506 mL	5.011 mL	25.056 mL	50.113 mL
5 mM	100.226 µL	501.128 µL	1.002 mL	5.011 mL	10.023 mL
10 mM	50.113 µL	250.564 µL	501.128 µL	2.506 mL	5.011 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	199.55 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Comparison among different “revealers” in the study of accelerated blood clearance phenomenon

Authors:

Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui

Journal:

European Journal of Pharmaceutical Sciences (2018): 210--216

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy

Authors:

Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao

Journal:

Colloids and Surfaces B: Biointerfaces (2017)

References

View all 102 references: Citation Explorer

Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles

Authors:

Cheng Z, Aspinwall CA.

Journal:

Analyst (2006): 236

The two ATP binding sites of cystic fibrosis transmembrane conductance regulator (CFTR) play distinct roles in gating kinetics and energetics

Authors:

Zhou Z, Wang X, Liu HY, Zou X, Li M, Hwang TC.

Journal:

J Gen Physiol (2006): 413

Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens

Authors:

Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.

Journal:

Mol Hum Reprod (2006): 717

In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer

Authors:

Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.

Journal:

Embo J (2006): 4728

Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent

Authors:

Higashi Y, Nakamura S, Matsumura H, Fujii Y.

Journal:

Biomed Chromatogr (2006): 423

Physical properties

Molecular weight	199.55
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000
Excitation (nm)	467
Emission (nm)	539

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	10199-89-0

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