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NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*

NBD-Cl is widely used to label peptides, proteins, drugs and other biomolecules. It is also a popular derivatizing reagent for HPLC analysis. NBD-Cl is non-fluorescent, and generates fluorescent adducts upon reacting with aliphatic amines or thiol compounds. It reacts with amino groups such as aliphatic amines, amino acids, peptides, and proteins to form highly fluorescent compounds. The NBD-amine adducts have ?ex = 464 nm and ?em = 512 nm in aqueous solutions. The fluorescence spectra of NBD-amine adducts are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions. NBD-Cl also reacts with thiol group to form fluorescent adducts, but the thiol adducts are much less fluorescent than the amine adducts.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM501.128 µL2.506 mL5.011 mL25.056 mL50.113 mL
5 mM100.226 µL501.128 µL1.002 mL5.011 mL10.023 mL
10 mM50.113 µL250.564 µL501.128 µL2.506 mL5.011 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
NBD-F [4-Fluoro-7-nitrobenzofurazan] *CAS 29270-56-2*46753922000
CL-APC [Cross linked-AlloPhycocyanin]651660730000

Citations

View all 2 citations: Citation Explorer
Comparison among different “revealers” in the study of accelerated blood clearance phenomenon
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

References

View all 102 references: Citation Explorer
Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles
Authors: Cheng Z, Aspinwall CA.
Journal: Analyst (2006): 236
The two ATP binding sites of cystic fibrosis transmembrane conductance regulator (CFTR) play distinct roles in gating kinetics and energetics
Authors: Zhou Z, Wang X, Liu HY, Zou X, Li M, Hwang TC.
Journal: J Gen Physiol (2006): 413
Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens
Authors: Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.
Journal: Mol Hum Reprod (2006): 717
In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer
Authors: Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.
Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
Authors: Higashi Y, Nakamura S, Matsumura H, Fujii Y.
Journal: Biomed Chromatogr (2006): 423
Page updated on November 5, 2024

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Physical properties

Molecular weight

199.55

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

22000

Excitation (nm)

467

Emission (nm)

539

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

10199-89-0
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