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NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0*
NBD-Cl is widely used to label peptides, proteins, drugs and other biomolecules. It is also a popular derivatizing reagent for HPLC analysis. NBD-Cl is non-fluorescent, and generates fluorescent adducts upon reacting with aliphatic amines or thiol compounds. It reacts with amino groups such as aliphatic amines, amino acids, peptides, and proteins to form highly fluorescent compounds. The NBD-amine adducts have ?ex = 464 nm and ?em = 512 nm in aqueous solutions. The fluorescence spectra of NBD-amine adducts are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions. NBD-Cl also reacts with thiol group to form fluorescent adducts, but the thiol adducts are much less fluorescent than the amine adducts.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 501.128 µL | 2.506 mL | 5.011 mL | 25.056 mL | 50.113 mL |
| 5 mM | 100.226 µL | 501.128 µL | 1.002 mL | 5.011 mL | 10.023 mL |
| 10 mM | 50.113 µL | 250.564 µL | 501.128 µL | 2.506 mL | 5.011 mL |
Molarity calculator
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Citations
View all 2 citations: Citation Explorer
Comparison among different “revealers” in the study of accelerated blood clearance phenomenon
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
References
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Authors: Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.
Journal: Mol Hum Reprod (2006): 717
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Authors: Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.
Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
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Authors: Higashi Y, Nakamura S, Matsumura H, Fujii Y.
Journal: Biomed Chromatogr (2006): 423
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