NBD-F [4-Fluoro-7-nitrobenzofurazan] *CAS 29270-56-2*

NBD-F

4-Fluoro-7-nitrobenzofurazan; CAS 29270-56-2

NBD-F has properties and applications similar to those of NBD-Cl. Upon reacting with amines and thiol compounds it generates the same fluorescent adducts as those of NBD-Cl. Compared with NBD-Cl, NBD-F is much more reactive, and should be more carefully stored. For example, the reaction of NBD fluoride with glycine is reported to be 500 times faster than the reaction of NBD chloride with glycine. Both NBD chloride and NBD fluoride are extensively used as derivatization reagents for chromatographic analysis of amino acids and other low molecular weight amines.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
821	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-F [4-Fluoro-7-nitrobenzofurazan] *CAS 29270-56-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	546.15 µL	2.731 mL	5.461 mL	27.307 mL	54.615 mL
5 mM	109.23 µL	546.15 µL	1.092 mL	5.461 mL	10.923 mL
10 mM	54.615 µL	273.075 µL	546.15 µL	2.731 mL	5.461 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	183.1 g/mol	=	mL	x	mM	=	mmol

References

View all 102 references: Citation Explorer

Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles

Authors:

Cheng Z, Aspinwall CA.

Journal:

Analyst (2006): 236

The two ATP binding sites of cystic fibrosis transmembrane conductance regulator (CFTR) play distinct roles in gating kinetics and energetics

Authors:

Zhou Z, Wang X, Liu HY, Zou X, Li M, Hwang TC.

Journal:

J Gen Physiol (2006): 413

Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens

Authors:

Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.

Journal:

Mol Hum Reprod (2006): 717

In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer

Authors:

Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.

Journal:

Embo J (2006): 4728

Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent

Authors:

Higashi Y, Nakamura S, Matsumura H, Fujii Y.

Journal:

Biomed Chromatogr (2006): 423

Physical properties

Molecular weight	183.1
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000
Excitation (nm)	467
Emission (nm)	539

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	29270-56-2

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025