Name: NBD-PE [N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt]
Brand: AAT Bioquest
SKU: 23305
Price: 179 USD
Availability: InStock

NBD-PE

N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt

NBD-PE is a fluorescent phospholipid that integrates into cellular membranes with environment-sensitive fluorescence, enabling studies of membrane dynamics, lipid trafficking, and membrane fusion events.

Environment-Sensitive Fluorophore: NBD head group enables fluorescence changes based on membrane environment
Membrane Integration: Efficiently incorporates into lipid bilayers for dynamic studies
Versatile Applications: Ideal for studying membrane dynamics, fusion, and lipid interactions

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23305	5 mg	Price

Overview

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a fluorescent phospholipid commonly used in cell biology research for studying membrane dynamics, including membrane fluidity and trafficking. Its ability to integrate seamlessly into cellular membranes and fluoresce upon excitation makes it a valuable tool for investigating the physical and functional properties of lipid bilayers.

This phospholipid is modified at its head group with the environment-sensitive fluorophore NBD, which has excitation and emission maxima at approximately 467 nm and 539 nm, respectively. When paired with complementary dyes such as Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027), NBD-PE enables fluorescence resonance energy transfer (FRET)-based assays for studying membrane fusion events. Furthermore, it has been widely applied in fluorescence recovery after photobleaching (FRAP) experiments to quantitatively assess membrane dynamics and lipid mobility.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-PE [N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.576 µL	522.881 µL	1.046 mL	5.229 mL	10.458 mL
5 mM	20.915 µL	104.576 µL	209.153 µL	1.046 mL	2.092 mL
10 mM	10.458 µL	52.288 µL	104.576 µL	522.881 µL	1.046 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	956.24 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

ATG9B is a tissue-specific homotrimeric lipid scramblase that can compensate for ATG9A.

Authors:

Chiduza, George N and Garza-Garcia, Acely and Almacellas, Eugenia and De Tito, Stefano and Pye, Valerie E and van Vliet, Alexander R and Cherepanov, Peter and Tooze, Sharon A

Journal:

Autophagy (2024): 557-576

Flow-based bioconjugation of coumarin phosphatidylethanolamine probes: Optimised synthesis and membrane molecular dynamics studies.

Authors:

Varandas, Pedro A M M and Belinha, Ricardo and Cobb, Alexander J A and Prates Ramalho, João P and Segundo, Marcela A and Loura, Luís M S and Silva, Eduarda M P

Journal:

Biochimica et biophysica acta. Biomembranes (2024): 184335

Fluorescent hydrophobic ion pairs: A powerful tool to investigate cellular uptake of hydrophobic drug complexes via lipid-based nanocarriers.

Authors:

Zöller, Katrin and Karlegger, Anna and Truszkowska, Martyna and Stengel, Daniel and Bernkop-Schnürch, Andreas

Journal:

Journal of colloid and interface science (2024): 174-188

--Atg9 interactions via its transmembrane domains are required for phagophore expansion during autophagy.

Authors:

Chumpen Ramirez, Sabrina and Gómez-Sánchez, Rubén and Verlhac, Pauline and Hardenberg, Ralph and Margheritis, Eleonora and Cosentino, Katia and Reggiori, Fulvio and Ungermann, Christian

Journal:

Autophagy (2023): 1459-1478

A Limitation of Using Dithionite Quenching to Determine the Topology of Membrane-inserted Proteins.

Authors:

O'Neil, Pierce T

Journal:

The Journal of membrane biology (2022): 123-127

Physical properties

Molecular weight	956.24
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000
Excitation (nm)	467
Emission (nm)	539

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	178119-00-1

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 20, 2025