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NBD-X, succinimidyl ester *CAS 145195-58-0*

Compared to NBD chloride and fluoride, NBD-X acid gives better yield for labeling biopolymers. The fluorescence spectra of NBD derivatives are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-X, succinimidyl ester *CAS 145195-58-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM255.539 µL1.278 mL2.555 mL12.777 mL25.554 mL
5 mM51.108 µL255.539 µL511.078 µL2.555 mL5.111 mL
10 mM25.554 µL127.769 µL255.539 µL1.278 mL2.555 mL

Molarity calculator

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Spectrum

Citations

View all 2 citations: Citation Explorer
Comparison among different “revealers” in the study of accelerated blood clearance phenomenon
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

References

View all 102 references: Citation Explorer
Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles
Authors: Cheng Z, Aspinwall CA.
Journal: Analyst (2006): 236
The two ATP binding sites of cystic fibrosis transmembrane conductance regulator (CFTR) play distinct roles in gating kinetics and energetics
Authors: Zhou Z, Wang X, Liu HY, Zou X, Li M, Hwang TC.
Journal: J Gen Physiol (2006): 413
Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens
Authors: Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.
Journal: Mol Hum Reprod (2006): 717
In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer
Authors: Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.
Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
Authors: Higashi Y, Nakamura S, Matsumura H, Fujii Y.
Journal: Biomed Chromatogr (2006): 423
Page updated on October 28, 2024

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Physical properties

Molecular weight

391.33

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

22000

Excitation (nm)

467

Emission (nm)

539

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

145195-58-0
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