Name: Newport Green™ DCF Diacetate *Cell Permeant*
Brand: AAT Bioquest
SKU: 21265
Price: 295 USD
Availability: InStock

Newport Green™ DCF Diacetate

Cell Permeant

Newport Green™ DCF Diacetate is a cell-permeable zinc indicator that selectively detects intracellular Zn²⁺ with moderate affinity, ideal for monitoring zinc flux in neurons and excitable cells.

Zinc-Selective Detection: Moderate Zn²⁺ affinity (K_d ≈ 1 μM) with minimal Ca²⁺ cross-reactivity for specific monitoring
Stable Intracellular Retention: Water-soluble de-esterified form remains in cytoplasm with minimal leakage
FITC Filter Compatible: Excitation/emission at 505/535 nm works with standard green fluorescence channels
FluoZin-3 Alternative: More cell-permeable than FluoZin indicators with comparable zinc sensitivity

The chemical structure of Newport Green™ DCF Diacetate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21265	1 mg	Price

Overview

Newport Green™ DCF Diacetate is a cell-permeable zinc indicator optimized for the quantitative detection of intracellular Zn²⁺ in live-cell systems. In its diacetate-protected form, the probe passively diffuses across the plasma membrane. Upon cellular entry, endogenous cytosolic esterases hydrolyze the acetyl groups, generating the de-esterified Newport Green™ DCF dye, which is water-soluble and effectively retained within the cytoplasm. The activated dye exhibits a moderate affinity for Zn²⁺ (K_d ≈ 1 μM), enabling sensitive detection of physiologically relevant concentrations of labile zinc. Importantly, it displays minimal affinity for Ca²⁺ (K_d > 100 μM), providing high selectivity for Zn²⁺ over other intracellular divalent cations. This selectivity makes it particularly suitable for investigating dynamic Zn²⁺ signaling events in excitable cells, such as neurons, where zinc influx is mediated by glutamatergic stimulation or voltage-gated ion channels. Newport Green™ DCF exhibits excitation and emission maxima at approximately 505 nm and 535 nm, respectively, ensuring compatibility with standard FITC filter sets for applications including fluorescence microscopy, flow cytometry, and high-content imaging.

References

View all 50 references: Citation Explorer

Zinc accumulation aggravates cerebral ischemia/reperfusion injury by promoting inflammation.

Authors:

Li, Wei and Yang, Xueqi and Ding, Mao and Shi, Wenjuan and Huang, Yuyou and An, Qi and Qi, Zhifeng and Zhao, Yongmei

Journal:

Frontiers in cellular neuroscience (2023): 1065873

Transmembrane 163 (TMEM163) protein interacts with specific mammalian SLC30 zinc efflux transporter family members.

Authors:

Escobar, Adrian and Styrpejko, Daniel J and Ali, Saima and Cuajungco, Math P

Journal:

Biochemistry and biophysics reports (2022): 101362

Autophagy Involves in Differentiation of Insulin-Secreting Cells from Adipose Derived Stem Cells.

Authors:

Hashemitabar, Mahmoud and Rezaei-Tazangi, Fatemeh and Khorsandi, Layasadat and Mard, Seyed Ali

Journal:

Cell journal (2021): 619-625

A new zinc chelator, IPZ-010 ameliorates postoperative ileus.

Authors:

Kimura, Hitomi and Yoneya, Yutaka and Mikawa, Shoma and Kaji, Noriyuki and Ito, Hiroki and Tsuchida, Yasuaki and Komatsu, Hirotsugu and Murata, Takahisa and Ozaki, Hiroshi and Uchida, Ryota and Nishida, Keigo and Hori, Masatoshi

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2020): 109773

Zn2+ and mPTP mediate resveratrol-induced myocardial protection from endoplasmic reticulum stress.

Authors:

He, Yifei and Fu, Yu and Xi, Mengyao and Zheng, Huan and Zhang, Yidong and Liu, Yulin and Zhao, Yang and Xi, Jinkun and He, Yonggui

Journal:

Metallomics : integrated biometal science (2020): 290-300

Physical properties

Molecular weight	801.64
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	792895-35-3

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Page updated on October 3, 2025