Nile Blue A chloride
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 353.85 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 631 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
CAS 2381-85-3 | Molecular weight 353.85 | Absorbance (nm) 631 |
Nile blue A is a stain used in biology and histology. It may be used with live or fixed cells and imparts a blue color to cell nuclei. It is used in a variety of commercial DNA color staining formulations for DNA electrophoresis. As it does not require UV trans-illumination for visualization in an agarose gel as with ethidium bromide and other fluorescent stains such SYBR Green or Gel Red, it can be used to observe DNA as it is separated and also as a dye to aid in gel-extraction of DNA fragments without incurring damage by UV-irradiation. The derivatives of Nile Blue A are potential photosensitizers in photodynamic therapy of malignant tumors. These dyes aggregate in the tumor cells, especially in the lipid membranes, and/or are sequestered and concentrated in subcellular organelles.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Blue A chloride to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 282.606 µL | 1.413 mL | 2.826 mL | 14.13 mL | 28.261 mL |
5 mM | 56.521 µL | 282.606 µL | 565.211 µL | 2.826 mL | 5.652 mL |
10 mM | 28.261 µL | 141.303 µL | 282.606 µL | 1.413 mL | 2.826 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 50 references: Citation Explorer
In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions.
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Journal: Scientific reports (2020): 3752
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Journal: Scientific reports (2020): 3752
Ratiometric Strategy for Electrochemical Sensing of Carbaryl Residue in Water and Vegetable Samples.
Authors: Zhang, Min and Zhang, Zeyuan and Yang, Yanjing and Zhang, Yi and Wang, Yufei and Chen, Xinyi
Journal: Sensors (Basel, Switzerland) (2020)
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A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.
Authors: Duan, Nuo and Shen, Mofei and Qi, Shuo and Wang, Wenyue and Wu, Shijia and Wang, Zhouping
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Authors: Duan, Nuo and Shen, Mofei and Qi, Shuo and Wang, Wenyue and Wu, Shijia and Wang, Zhouping
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Development of novel optical pH sensors based on coumarin 6 and nile blue A encapsulated in resin particles and specific support materials.
Authors: Duong, Hong Dinh and Shin, Younsook and Rhee, Jong Il
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110323
Authors: Duong, Hong Dinh and Shin, Younsook and Rhee, Jong Il
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110323
SERS-Based Immunoassays for the Detection of Botulinum Toxins A and B Using Magnetic Beads.
Authors: Kim, Kihyun and Choi, Namhyun and Jeon, Jun Ho and Rhie, Gi-Eun and Choo, Jaebum
Journal: Sensors (Basel, Switzerland) (2019)
Authors: Kim, Kihyun and Choi, Namhyun and Jeon, Jun Ho and Rhie, Gi-Eun and Choo, Jaebum
Journal: Sensors (Basel, Switzerland) (2019)
Silver-nanoparticles-loaded chitosan foam as a flexible SERS substrate for active collecting analytes from both solid surface and solution.
Authors: Wang, Cong and Wong, Ka Wai and Wang, Qun and Zhou, Yufeng and Tang, Changyu and Fan, Meikun and Mei, Jun and Lau, Woon-Ming
Journal: Talanta (2019): 241-247
Authors: Wang, Cong and Wong, Ka Wai and Wang, Qun and Zhou, Yufeng and Tang, Changyu and Fan, Meikun and Mei, Jun and Lau, Woon-Ming
Journal: Talanta (2019): 241-247
pH-Sensitive Chitosan-Sodium Phytate Core-Shell Hollow Beads and Nanocapsules for the Encapsulation of Active Ingredients.
Authors: Yang, Jie and Wang, Yanfei and Li, Man and Wu, Hao and Zhen, Tianyuan and Xiong, Liu and Sun, Qingjie
Journal: Journal of agricultural and food chemistry (2019): 2894-2905
Authors: Yang, Jie and Wang, Yanfei and Li, Man and Wu, Hao and Zhen, Tianyuan and Xiong, Liu and Sun, Qingjie
Journal: Journal of agricultural and food chemistry (2019): 2894-2905
Preparation and characterization of redox-sensitive glutenin nanoparticles.
Authors: Li, Fang and Qiu, Chao and Li, Man and Xiong, Liu and Shi, Yanping and Sun, Qingjie
Journal: International journal of biological macromolecules (2019): 327-336
Authors: Li, Fang and Qiu, Chao and Li, Man and Xiong, Liu and Shi, Yanping and Sun, Qingjie
Journal: International journal of biological macromolecules (2019): 327-336
Visualization of viral DNA assembly using nonfluorescent staining.
Authors: Karalova, E M and Hakobyan, L H and Tatoyan, M R and Sahakyan, K T and Karalyan, Z A
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2019): 309-312
Authors: Karalova, E M and Hakobyan, L H and Tatoyan, M R and Sahakyan, K T and Karalyan, Z A
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2019): 309-312
Mussel-Inspired Fabrication of SERS Swabs for Highly Sensitive and Conformal Rapid Detection of Thiram Bactericides.
Authors: Liu, Jun and Si, Tiantian and Zhang, Lingzi and Zhang, Zhiliang
Journal: Nanomaterials (Basel, Switzerland) (2019)
Authors: Liu, Jun and Si, Tiantian and Zhang, Lingzi and Zhang, Zhiliang
Journal: Nanomaterials (Basel, Switzerland) (2019)