Nile Blue A chloride

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	353.85
Solvent	DMSO

Spectral properties

Absorbance (nm)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

2381-85-3

Molecular weight

353.85

Absorbance (nm)

631

Nile blue A is a stain used in biology and histology. It may be used with live or fixed cells and imparts a blue color to cell nuclei. It is used in a variety of commercial DNA color staining formulations for DNA electrophoresis. As it does not require UV trans-illumination for visualization in an agarose gel as with ethidium bromide and other fluorescent stains such SYBR Green or Gel Red, it can be used to observe DNA as it is separated and also as a dye to aid in gel-extraction of DNA fragments without incurring damage by UV-irradiation. The derivatives of Nile Blue A are potential photosensitizers in photodynamic therapy of malignant tumors. These dyes aggregate in the tumor cells, especially in the lipid membranes, and/or are sequestered and concentrated in subcellular organelles.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Blue A chloride to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	282.606 µL	1.413 mL	2.826 mL	14.13 mL	28.261 mL
5 mM	56.521 µL	282.606 µL	565.211 µL	2.826 mL	5.652 mL
10 mM	28.261 µL	141.303 µL	282.606 µL	1.413 mL	2.826 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

631

Images

Figure 1. Chemical structure for Nile Blue A chloride.

References

View all 50 references: Citation Explorer

In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions.
Authors: Sruamsiri, Donhatai and Thayanukul, Parinda and Suwannasilp, Benjaporn Boonchayaanant
Journal: Scientific reports (2020): 3752

Ratiometric Strategy for Electrochemical Sensing of Carbaryl Residue in Water and Vegetable Samples.
Authors: Zhang, Min and Zhang, Zeyuan and Yang, Yanjing and Zhang, Yi and Wang, Yufei and Chen, Xinyi
Journal: Sensors (Basel, Switzerland) (2020)

A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.
Authors: Duan, Nuo and Shen, Mofei and Qi, Shuo and Wang, Wenyue and Wu, Shijia and Wang, Zhouping
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118103

Development of novel optical pH sensors based on coumarin 6 and nile blue A encapsulated in resin particles and specific support materials.
Authors: Duong, Hong Dinh and Shin, Younsook and Rhee, Jong Il
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110323

An investigation for recovery of polyhydroxyalkanoates (PHA) from Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19 isolated from plastic waste landfill.
Authors: Mohammed, Seid and Panda, Ananta N and Ray, Lopamudra
Journal: International journal of biological macromolecules (2019): 1085-1096

Mussel-Inspired Fabrication of SERS Swabs for Highly Sensitive and Conformal Rapid Detection of Thiram Bactericides.
Authors: Liu, Jun and Si, Tiantian and Zhang, Lingzi and Zhang, Zhiliang
Journal: Nanomaterials (Basel, Switzerland) (2019)

Visualization of viral DNA assembly using nonfluorescent staining.
Authors: Karalova, E M and Hakobyan, L H and Tatoyan, M R and Sahakyan, K T and Karalyan, Z A
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2019): 309-312

A vertical flow microarray chip based on SERS nanotags for rapid and ultrasensitive quantification of α-fetoprotein and carcinoembryonic antigen.
Authors: Zhang, Di and Huang, Li and Liu, Bing and Ge, Qinyu and Dong, Jian and Zhao, Xiangwei
Journal: Mikrochimica acta (2019): 699

Preparation and characterization of redox-sensitive glutenin nanoparticles.
Authors: Li, Fang and Qiu, Chao and Li, Man and Xiong, Liu and Shi, Yanping and Sun, Qingjie
Journal: International journal of biological macromolecules (2019): 327-336

pH-Sensitive Chitosan-Sodium Phytate Core-Shell Hollow Beads and Nanocapsules for the Encapsulation of Active Ingredients.
Authors: Yang, Jie and Wang, Yanfei and Li, Man and Wu, Hao and Zhen, Tianyuan and Xiong, Liu and Sun, Qingjie
Journal: Journal of agricultural and food chemistry (2019): 2894-2905