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Nonyl acridine orange *CAS 75168-11-5*
10-n-Nonyl acridine orange (NAO) is a mitochondrial-specific fluorochrome. In contrast with Rh 123, NAO accumulation in the cell does not seem to be driven by the proton-motrice force but does seem to be related to specific interactions with mitochondrial membrane proteins and/or lipids. NAO uptake is independent of mitochondrial transmembrane potential. It is used to examine the changes in the mitochondrial compartment during the transfer of articular chondrocytes from cartilage to the culture conditions, where Rh 123 proves changes in mitochondrial activity and/or biogenesis. It is used for distinguishing mitochondrial activity from biogenesis. NAO and Rhodamine 123 (Rh 123) is a good pair of dyes for studying mitochondrial functions.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nonyl acridine orange *CAS 75168-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 211.64 µL | 1.058 mL | 2.116 mL | 10.582 mL | 21.164 mL |
| 5 mM | 42.328 µL | 211.64 µL | 423.28 µL | 2.116 mL | 4.233 mL |
| 10 mM | 21.164 µL | 105.82 µL | 211.64 µL | 1.058 mL | 2.116 mL |
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References
View all 15 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin
Authors: Gohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML.
Journal: Anal Biochem (2005): 350
Authors: Gohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML.
Journal: Anal Biochem (2005): 350
Determination of dipole moment in the ground and excited state by experimental and theoretical methods of N-nonyl acridine orange
Authors: Wiosetek-Reske AM, Wysocki S, Bak GW.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1172
Authors: Wiosetek-Reske AM, Wysocki S, Bak GW.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1172
Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models
Authors: Garcia Fern, undefined and ez MI, Ceccarelli D, Muscatello U.
Journal: Anal Biochem (2004): 174
Authors: Garcia Fern, undefined and ez MI, Ceccarelli D, Muscatello U.
Journal: Anal Biochem (2004): 174
Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass
Authors: Jacobson J, Duchen MR, Heales SJ.
Journal: J Neurochem (2002): 224
Authors: Jacobson J, Duchen MR, Heales SJ.
Journal: J Neurochem (2002): 224
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