Nonyl acridine orange *CAS 75168-11-5*

Nonyl acridine orange

CAS 75168-11-5

10-n-Nonyl acridine orange (NAO) is a mitochondrial-specific fluorochrome. In contrast with Rh 123, NAO accumulation in the cell does not seem to be driven by the proton-motrice force but does seem to be related to specific interactions with mitochondrial membrane proteins and/or lipids. NAO uptake is independent of mitochondrial transmembrane potential. It is used to examine the changes in the mitochondrial compartment during the transfer of articular chondrocytes from cartilage to the culture conditions, where Rh 123 proves changes in mitochondrial activity and/or biogenesis. It is used for distinguishing mitochondrial activity from biogenesis. NAO and Rhodamine 123 (Rh 123) is a good pair of dyes for studying mitochondrial functions.

Catalog	Size	Price	Quantity
22205	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nonyl acridine orange *CAS 75168-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.64 µL	1.058 mL	2.116 mL	10.582 mL	21.164 mL
5 mM	42.328 µL	211.64 µL	423.28 µL	2.116 mL	4.233 mL
10 mM	21.164 µL	105.82 µL	211.64 µL	1.058 mL	2.116 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	472.5 g/mol	=	mL	x	mM	=	mmol

References

View all 15 references: Citation Explorer

Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants

Authors:

Wiosetek-Reske AM, Wysocki S.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118

Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin

Authors:

Gohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML.

Journal:

Anal Biochem (2005): 350

Determination of dipole moment in the ground and excited state by experimental and theoretical methods of N-nonyl acridine orange

Authors:

Wiosetek-Reske AM, Wysocki S, Bak GW.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2005): 1172

Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models

Authors:

Garcia Fern, undefined and ez MI, Ceccarelli D, Muscatello U.

Journal:

Anal Biochem (2004): 174

Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass

Authors:

Jacobson J, Duchen MR, Heales SJ.

Journal:

J Neurochem (2002): 224

Physical properties

Molecular weight	472.5
Solvent	DMSO

Spectral properties

Excitation (nm)	496
Emission (nm)	519

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	75168-11-5

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