Name: NTA maleimide
Brand: AAT Bioquest
SKU: 12609
Price: 115 USD
Availability: InStock

NTA maleimide

NTA maleimide is used to attach NTA groups to a biological substrate or a surface that has thiol groups. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method. The NTA maleimide is an excellent building block for developing the HIS tag detection probes.

Catalog	Size	Price	Quantity
12609	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NTA maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	241.908 µL	1.21 mL	2.419 mL	12.095 mL	24.191 mL
5 mM	48.382 µL	241.908 µL	483.816 µL	2.419 mL	4.838 mL
10 mM	24.191 µL	120.954 µL	241.908 µL	1.21 mL	2.419 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	413.38 g/mol	=	mL	x	mM	=	mmol

References

View all 14 references: Citation Explorer

Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)

Authors:

de Costa F, Barber CJ, Pujara PT, Reed DW, Covello PS.

Journal:

Methods Mol Biol (2016): 91

New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli

Authors:

Schwendener S, Perreten V.

Journal:

Appl Environ Microbiol (2015): 3243

Characterization of soluble RNA-dependent RNA polymerase from dengue virus serotype 2: The polyhistidine tag compromises the polymerase activity

Authors:

Kamkaew M, Chimnaronk S.

Journal:

Protein Expr Purif (2015): 43

Cellular uptake and in vivo distribution of polyhistidine peptides

Authors:

Iwasaki T, Tokuda Y, Kotake A, Okada H, Takeda S, Kawano T, Nakayama Y.

Journal:

J Control Release (2015): 115

Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis

Authors:

Panek A, Pietrow O, Filipkowski P, Synowiecki J.

Journal:

Acta Biochim Pol (2013): 163

Physical properties

Molecular weight	413.38
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	935666-21-0

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 28, 2025