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NTA maleimide
NTA maleimide is used to attach NTA groups to a biological substrate or a surface that has thiol groups. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method. The NTA maleimide is an excellent building block for developing the HIS tag detection probes.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of NTA maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 241.908 µL | 1.21 mL | 2.419 mL | 12.095 mL | 24.191 mL |
| 5 mM | 48.382 µL | 241.908 µL | 483.816 µL | 2.419 mL | 4.838 mL |
| 10 mM | 24.191 µL | 120.954 µL | 241.908 µL | 1.21 mL | 2.419 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 2 citations: Citation Explorer
Transition Metal Ion FRET-Based Probe to Study Cu (II)-Mediated Amyloid-$\beta$ Ligand Binding
Authors: Wu, Ri and Svingou, Despoina and Metternich, Jonas B and Benzenberg, Lukas R and Zenobi, Renato
Journal: Journal of the American Chemical Society (2024)
Authors: Wu, Ri and Svingou, Despoina and Metternich, Jonas B and Benzenberg, Lukas R and Zenobi, Renato
Journal: Journal of the American Chemical Society (2024)
References
View all 14 references: Citation Explorer
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Authors: de Costa F, Barber CJ, Pujara PT, Reed DW, Covello PS.
Journal: Methods Mol Biol (2016): 91
Authors: de Costa F, Barber CJ, Pujara PT, Reed DW, Covello PS.
Journal: Methods Mol Biol (2016): 91
New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli
Authors: Schwendener S, Perreten V.
Journal: Appl Environ Microbiol (2015): 3243
Authors: Schwendener S, Perreten V.
Journal: Appl Environ Microbiol (2015): 3243
Characterization of soluble RNA-dependent RNA polymerase from dengue virus serotype 2: The polyhistidine tag compromises the polymerase activity
Authors: Kamkaew M, Chimnaronk S.
Journal: Protein Expr Purif (2015): 43
Authors: Kamkaew M, Chimnaronk S.
Journal: Protein Expr Purif (2015): 43
Cellular uptake and in vivo distribution of polyhistidine peptides
Authors: Iwasaki T, Tokuda Y, Kotake A, Okada H, Takeda S, Kawano T, Nakayama Y.
Journal: J Control Release (2015): 115
Authors: Iwasaki T, Tokuda Y, Kotake A, Okada H, Takeda S, Kawano T, Nakayama Y.
Journal: J Control Release (2015): 115
Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis
Authors: Panek A, Pietrow O, Filipkowski P, Synowiecki J.
Journal: Acta Biochim Pol (2013): 163
Authors: Panek A, Pietrow O, Filipkowski P, Synowiecki J.
Journal: Acta Biochim Pol (2013): 163
Page updated on May 19, 2025
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