NTA succinimidyl ester

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) is such a ligand commonly used in commercially available resins for purifying proteins. On the other hand, tag-NTA conjugates are used for detecting polyhistidine-tagged proteins. NTA succinimidyl ester is an excellent building block that can be readily used to attach NTA groups to a biological substrate or a surface that has amino groups. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12607	1 mg	Price
12608	5 mg	Price

References

View all 31 references: Citation Explorer

Proteomics analysis of host cell proteins after immobilized metal affinity chromatography: Influence of ligand and metal ions.

Authors:

Lingg, Nico and Öhlknecht, Christoph and Fischer, Andreas and Mozgovicz, Markus and Scharl, Theresa and Oostenbrink, Chris and Jungbauer, Alois

Journal:

Journal of chromatography. A (2020): 461649

Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.

Authors:

Liu, Guizhen and Fu, Tao and Han, Ying and Hu, Shichen and Zhang, Xuepei and Zheng, Mengmeng and Hao, Piliang and Pan, Lifeng and Kang, Jingwu

Journal:

Analytical chemistry (2020): 3913-3922

Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system.

Authors:

Yang, Yuanzhong and Mitri, Khosse and Zhang, Chunfang and Boysen, Reinhard I and Hearn, Milton T W

Journal:

Protein expression and purification (2019): 51-61

Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.

Authors:

Kokhan, Oleksandr and Marzolf, Daniel R

Journal:

Analytical biochemistry (2019): 113347

Complementary IMAC enrichment methods for HLA-associated phosphopeptide identification by mass spectrometry.

Authors:

Abelin, Jennifer G and Trantham, Paisley D and Penny, Sarah A and Patterson, Andrea M and Ward, Stephen T and Hildebrand, William H and Cobbold, Mark and Bai, Dina L and Shabanowitz, Jeffrey and Hunt, Donald F

Journal:

Nature protocols (2015): 1308-18

Physical properties

Molecular weight	515.52
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 7, 2025