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Nuclear Black™ LCS1 *Cell-Permeable*
Product key features
Nuclear Black™ LCS1 is the first specialized reagent designed to suppress green nuclear fluorescence from live cell nucleus in fluorescence imaging experiments.
- Effective fluorescence suppression: Reduces unwanted green fluorescence from the nucleus of intact live cells to enhance signal-to-noise ratio in imaging
- Cell-permeable formulation: Penetrates live cells to suppress excess nuclear signal while preserving other fluorescence readouts
- Optimized for green channels: Ideal for experiments using green fluorescent probes or dyes
- Purpose-built innovation: Designed specifically to reduce green nuclear background from live-cell stains
Product description
Nuclear Black™ LCS1 is a novel cell permeable, fluorescence suppression reagent designed to reduce green nuclear background from live cells in fluorescence imaging experiments. Some commonly used cellular stains may cause excessive or non-specific nuclear fluorescence in live-cell assays. Nuclear Black™ LCS1 permeates intact cells and suppresses undesired green nuclear signals, improving overall image contrast and clarity.
Nuclear Black™ LCS1 is ideal for live-cell fluorescence microscopy, high-content screening, and imaging workflows where high background from nuclear staining in live cells interferes with signal interpretation. Its cell-permeable formulation integrates seamlessly into existing protocols to enhance green fluorescence visualization without affecting the target readout.
Example protocol
AT A GLANCE
- Prepare cells in growth medium.
- Stain cells with dye for live cells.
- Incubate cells with Nuclear Black™ LCS1 working solution at 37°C for 30 minutes.
- Analyze under fluorescence microscope with FITC filter set.
CELL PREPARATION
Plate cells overnight in growth medium at 10,000 to 40,000 cells/well/100 μL for a 96-well plate or 2,500 to 10,000 cells/well/50 μL for a 384-well plate.
PREPARATION OF WORKING SOLUTION
Note: The provided stock solution is 100X.
Note: Unused Nuclear Black™ LCS1 stock solution can be aliquoted and stored at ≤ -20 ºC for several months if the tubes are sealed tightly. Protect from light and avoid repeated freeze-thaw cycles.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare the cell samples and treat cells as desired.
- Optional: Remove the cell culture medium and wash cells with DPBS or buffer of your choice.
- Treat cells with live cells stain of your desire.
- Add 100 µL/well (96-well plate) or 50 µL/well (384-well plate) of Nuclear Black™ LCS1 working solution and incubate for 30 minutes at 37°C in a 5% CO2 incubator, protected from light.
Note: The optimal concentration of the Nuclear Black™ LCS1 varies depending on the specific applications. Concentration higher than the working solution can be toxic to cells. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
- Observe the fluorescence signal in cells using a fluorescence microscope with a FITC filter set.
Product family
| Name | Excitation (nm) | Emission (nm) |
| Nuclear Blue™ LCS1 | 353 | 456 |
| Nuclear Green™ LCS1 *5 mM DMSO Solution* | 503 | 527 |
| Nuclear Orange™ LCS1 *5 mM DMSO Solution* | 514 | 556 |
| Nuclear Red™ LCS1 *5 mM DMSO Solution* | 622 | 645 |
| Nuclear Violet™ LCS1 *5 mM DMSO Solution* | 401 | 460 |
Safety data sheet