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Nuclear Blue™ DCS2
Equivalent to SYTOX™ Blue Dead Cell Stain

Nuclear Blue™ DCS2 is the same molecule to SYTOX™ Blue Dead Cell Stain (SYTOX™ is a trademark of ThermoFisher). Nuclear Blue™ DCS2 is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. It is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. Under the same conditions, our Nuclear Violet™ DCS1 (#17549) gives much higher signal/background ratio than SYTOX™ Blue Dead Cell Stain. Nuclear Violet™ DCS1 is better excited by the violet laser at 405 nm than SYTOX™ Blue Dead Cell Stain. After brief incubation with Nuclear Violet™ DCS1 stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of Nuclear Violet™ DCS1 stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals.

A mixture of heat-killed and untreated Jurkat cells was stained with Nuclear Blue™ DCS2 (17546) or Nuclear Violet™ DCS1 (17549) stain for 10 minutes. Cells were analyzed on a flow cytometer equipped with a 405 nm violet laser and a 473/15 nm bandpass filter, such as the V4 channel on Cytek's Aurora spectral flow cytometer. Live cells are easily distinguished from the dead cell population.
A mixture of heat-killed and untreated Jurkat cells was stained with Nuclear Blue™ DCS2 (17546) or Nuclear Violet™ DCS1 (17549) stain for 10 minutes. Cells were analyzed on a flow cytometer equipped with a 405 nm violet laser and a 473/15 nm bandpass filter, such as the V4 channel on Cytek's Aurora spectral flow cytometer. Live cells are easily distinguished from the dead cell population.
CatalogSize
Price
Quantity
175460.5 ml
Price
175471 ml
Price
 
Physical properties

Molecular weight668.36
SolventDMSO
Spectral properties

Excitation (nm)445
Emission (nm)470
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings

Flow cytometer
Excitation405 nm Laser
Emission473/15 nm Filter
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Page updated on October 16, 2025