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Nuclear Blue™ LCS1

Our Nuclear Blue™ LCS1 is a fluorogenic, DNA-selective, and cell-permeant dye for analyzing DNA content in live cells. The Nuclear Blue™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for the multicolor analysis of live cells with the DAPI filter sets. For example, Nuclear Blue™LCS1 can be used with GFP cell lines.

Example protocol

AT A GLANCE

Spectral Properties

Ex/Em = 353/456 nm (bound to DNA)

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

  1. Add Nuclear Blue™ LCS1 (2 to10 µM) directly into the live cell culture medium (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

    Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.

  2. Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium. 

  3. Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set. 

Spectrum

References

View all 50 references: Citation Explorer
Breaking a Dogma: High-Throughput Live-Cell Imaging in Real-Time with Hoechst 33342.
Authors: Fuchs, Heiko and Jahn, Kirsten and Hu, Xiaonan and Meister, Roland and Binter, Maximilian and Framme, Carsten
Journal: Advanced healthcare materials (2023): e2300230
An annotated high-content fluorescence microscopy dataset with Hoechst 33342-stained nuclei and manually labelled outlines.
Authors: Arvidsson, Malou and Rashed, Salma Kazemi and Aits, Sonja
Journal: Data in brief (2023): 108769
Hoechst 33342 as a marker for imaging neurites of Dorsal Root Ganglion in vitro.
Authors: Merolli, Antonio and Bektas, Cemile
Journal: Journal of anatomy (2022): 998-1001
The pH and mercury ion stimuli-responsive supramolecular assemblies of cucurbit[7]uril and Hoechst 33342.
Authors: Wang, Qin and Lü, Li-Bing and Tao, Zhu and Sun, Tao and Tang, Qing and Huang, Ying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119656
Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP.
Authors: Swain, Brendan M and Guo, Dawei and Singh, Himansha and Rawlins, Philip B and McAlister, Mark and van Veen, Hendrik W
Journal: Scientific reports (2020): 20026
Page updated on December 11, 2024

Ordering information

Price
Unit size
Catalog Number17559
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Physical properties

Molecular weight

822.69

Solvent

DMSO

Spectral properties

Excitation (nm)

353

Emission (nm)

456

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

Platform

Fluorescence microscope

ExcitationDAPI Filter
EmissionDAPI Filter
Recommended plateBlack wall, clear bottom
Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.
Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.
Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.