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Nuclear Blue™ LCS1

Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.
Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.
Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.
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Physical properties
Molecular weight822.69
Spectral properties
Excitation (nm)353
Emission (nm)456
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Nuclear Blue™ LCS1 is a fluorogenic, DNA-selective, and cell-permeant dye for analyzing DNA content in live cells. The Nuclear Blue™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for the multicolor analysis of live cells with the DAPI filter sets. For example, Nuclear Blue™LCS1 can be used with GFP cell lines.


Fluorescence microscope

ExcitationDAPI Filter
EmissionDAPI Filter
Recommended plateBlack wall/clear bottom

Example protocol


Spectral Properties

Ex/Em = 353/456 nm (bound to DNA)


Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

  1. Add Nuclear Blue™ LCS1 (2 to10 µM) directly into the live cell culture medium (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

    Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.

  2. Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium. 

  3. Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set. 


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Blue™ LCS1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM121.552 µL607.762 µL1.216 mL6.078 mL12.155 mL
5 mM24.31 µL121.552 µL243.105 µL1.216 mL2.431 mL
10 mM12.155 µL60.776 µL121.552 µL607.762 µL1.216 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)353
Emission (nm)456



View all 50 references: Citation Explorer
Breaking a Dogma: High-Throughput Live-Cell Imaging in Real-Time with Hoechst 33342.
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Authors: Wang, Qin and Lü, Li-Bing and Tao, Zhu and Sun, Tao and Tang, Qing and Huang, Ying
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Authors: Swain, Brendan M and Guo, Dawei and Singh, Himansha and Rawlins, Philip B and McAlister, Mark and van Veen, Hendrik W
Journal: Scientific reports (2020): 20026
Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining.
Authors: Eddaoudi, Ayad and Canning, Stephanie Louise and Kato, Itaru
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 49-57
Photoselection of Luminescent Molecules in Anisotropic Media in the Case of Two-Photon Excitation. Part II. Experimental Studies of Hoechst 33342 in Stretched Poly(vinyl alcohol) Films.
Authors: Kawski, A and Gryczyński, Z and Gryczyński, I and Lakowicz, J R and Piszczek, G
Journal: Zeitschrift fur Naturforschung. A, Journal of physical sciences (2016): 1037-1041
Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage.
Authors: Schendzielorz, P and Froelich, K and Rak, K and Gehrke, T and Scherzad, A and Hagen, R and Radeloff, A
Journal: Stem cells international (2016): 6549347
Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and Propidium Iodide.
Authors: DeSouza, Ngoc and Zhou, Megan and Shan, Yi
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 47-57
Analyzing Cell Death by Nuclear Staining with Hoechst 33342.
Authors: Crowley, Lisa C and Marfell, Brooke J and Waterhouse, Nigel J
Journal: Cold Spring Harbor protocols (2016)