Nuclear Violet™ LCS1 5 mM DMSO Solution

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	401
Emission (nm)	460

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Molecular weight

N/A

Excitation (nm)

401

Emission (nm)

460

Our Nuclear Violet™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Violet™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~440 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live cells with the filter sets of Pacific Blue and BD Horizon V450.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	401
Emission (nm)	460

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Violet™ DCS1 5 mM DMSO Solution	371	454
Nuclear Green™ LCS1 5 mM DMSO Solution	503	527
Nuclear Orange™ LCS1 5 mM DMSO Solution	514	556
Nuclear Red™ LCS1 5 mM DMSO Solution	622	645
Nuclear Blue™ LCS1	353	456

Images

Figure 1. Fluorescence image of live HeLa cells stained with Nuclear Violet™ LCS1(Cat. 17543) and MitoLite™ Deep Red FX660 (Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.

Citations

View all 8 citations: Citation Explorer

Activation of invasion by oncogenic reprogramming of cholesterol metabolism via increased NPC1 expression and macropinocytosis
Authors: Skorda, Aikaterini and Lauridsen, Anna R{\o}ssberg and Wu, Chengnan and Huang, Jinrong and Mrackova, Monika and Winther, Nuggi Ingholt and Jank, Vanessa and Sztupinszki, Zsofia and Strauss, Robert and Bilgin, Mesut and others,
Journal: Oncogene (2023): 1--12

Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections
Authors: Honda, Mizuki and Kimura, Ryuichi and Harada, Akihito and Maehara, Kazumitsu and Tanaka, Kaori and Ohkawa, Yasuyuki and Oki, Shinya
Journal: STAR protocols (2022): 101346

Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs
Authors: Roberts, Brock and Hendershott, Melissa C and Arakaki, Joy and Gerbin, Kaytlyn A and Malik, Haseeb and Nelson, Angelique and Gehring, Jamie and Hookway, Caroline and Ludmann, Susan A and Yang, Ruian and others, undefined
Journal: Stem cell reports (2019)

Real-time 3D high-resolution microscopy of human cells on the international space station
Authors: Thiel, Cora Sandra and Tauber, Svantje and Seebacher, Christian and Schropp, Martin and Uhl, Rainer and Lauber, Beatrice and Polzer, Jennifer and Neelam, Srujana and Zhang, Ye and Ullrich, Oliver
Journal: International journal of molecular sciences (2019): 2033

Discovery of Orally Bioavailable, Quinoline-based Aldehyde Dehydrogenase 1A1 (ALDH1A1) Inhibitors with Potent Cellular Activity
Authors: Yang, Shyh-Ming and Martinez, Natalia J and Yasgar, Adam and Danchik, Carina and Johansson, Catrine and Wang, Yuhong and Baljinnyam, Bolormaa and Wang, Amy Q and Xu, Xin and Shah, Pranav and others, undefined
Journal: Journal of medicinal chemistry (2018)

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References

View all 65 references: Citation Explorer

In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751

Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534

Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706

Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555

Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93

Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759

Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84

DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154