Nuclear Green™ DCS1 5 mM DMSO Solution

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 594 Phalloidin (Cat#23122, Red) and nuclei stain Nuclear Green™ DCS1 (Cat#17550, Green)

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	503
Emission (nm)	527

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Nuclear Blue™ DCS1 *5 mM DMSO Solution*

Nuclear Orange™ DCS1 *5 mM DMSO Solution*

Nuclear Red™ DCS1 *5 mM DMSO Solution*

Overview SDS Protocol

Excitation (nm)

503

Emission (nm)

527

Our Nuclear Green™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Green™ DCS1 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with proper filter sets.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	503
Emission (nm)	527

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Blue™ DCS1 5 mM DMSO Solution	348	469
Nuclear Orange™ DCS1 5 mM DMSO Solution	514	556
Nuclear Red™ DCS1 5 mM DMSO Solution	631	651

Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 594 Phalloidin (Cat#23122, Red) and nuclei stain Nuclear Green™ DCS1 (Cat#17550, Green)

Citations

View all 5 citations: Citation Explorer

Oxidative Stress-and Autophagy-Inducing Effects of PSI-LHCI from Botryococcus braunii in Breast Cancer Cells
Authors: Joaqu{\'\i}n-Ovalle, Freisa M and Guihurt, Grace and Barcelo-Bovea, Vanessa and Hani-Saba, Andraous and Fontanet-G{\'o}mez, Nicole C and Ramirez-Paz, Josell and Kashino, Yasuhiro and Torres-Martinez, Zally and Doble-Cacho, Katerina and Delinois, Louis J and others,
Journal: BioTech (2022): 9

Investigating the Molecular Mechanisms of TMEM16F--a Ca2+ Activated Phospholipid Scramblase and Ion Channel
Authors: Le, Trieu Phuong Hai
Journal: (2021)

Evidence that polyphenols do not inhibit the phospholipid scramblase TMEM16F
Authors: Le, Trieu and Le, Son C and Zhang, Yang and Liang, Pengfei and Yang, Huanghe
Journal: Journal of Biological Chemistry (2020): 12537--12544

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References

View all 65 references: Citation Explorer

In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751

Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534

Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706

Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555

Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93

Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759

Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84

DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154