Nuclear Violet™ DCS1 5 mM DMSO Solution

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	744.77
Solvent	DMSO

Spectral properties

Excitation (nm)	371
Emission (nm)	454

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

744.77

Excitation (nm)

371

Emission (nm)

454

Our Nuclear Violet™ DCS1 is a fluorogenic, DNA-selective and live cell-impermeant dye for analyzing DNA content in dead or fixed cells. The Nuclear Violet™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~440 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live/dead cells with the filter sets of Pacific Blue and BD Horizon V450.

Platform

Fluorescence microscope

Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Example protocol

PREPARATION OF WORKING SOLUTION

Nuclear Violet™ DCS1 working solution

Dilute the Nuclear Violet™ DCS1 stock solution (5 mM) to 0.5 to 5 µM final concentration in the buffer of your choice.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.

Add Nuclear Violet™ DCS1 working solution into the fixed, dead or apoptotic cells (either suspension or adherent) and stain the cells for 15 to 60 minutes. In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result.
Directly analyze the cellular staining with fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
Note Optional: Cells can be washed with PBS prior to analysis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Violet™ DCS1 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	134.27 µL	671.348 µL	1.343 mL	6.713 mL	13.427 mL
5 mM	26.854 µL	134.27 µL	268.539 µL	1.343 mL	2.685 mL
10 mM	13.427 µL	67.135 µL	134.27 µL	671.348 µL	1.343 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	371
Emission (nm)	454

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Violet™ LCS1 5 mM DMSO Solution	401	460
Nuclear Blue™ DCS1 5 mM DMSO Solution	348	469
Nuclear Green™ DCS1 5 mM DMSO Solution	503	527
Nuclear Orange™ DCS1 5 mM DMSO Solution	514	556
Nuclear Red™ DCS1 5 mM DMSO Solution	631	651

Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde and then stained with Nuclear Violet™ DCS1.

Figure 2. Fixed and Live (non-fixed) HeLa cells were plated on 96-well plates, incubated with Nuclear Violet™ DCS1 1 µM for 20 minutes, and imaged with a DAPI channel.

References

View all 50 references: Citation Explorer

Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Cordova, Miguel and Kose, Kivanc and Aleissa, Saud and Alessi-Fox, Christi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of biophotonics (2021): e202000207

Assessment of laser-induced thermal damage in fresh skin with ex vivo confocal microscopy.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of the American Academy of Dermatology (2021): e19-e21

Development of a novel flow cytometry-based approach for reticulocytes micronucleus test in rat peripheral blood.
Authors: Chen, Yiyi and Huo, Jiao and Liu, Yunjie and Zeng, Zhu and Zhu, Xuejiao and Chen, Xuxi and Wu, Rui and Zhang, Lishi and Chen, Jinyao
Journal: Journal of applied toxicology : JAT (2021): 595-606

A novel method to purify neutrophils enables functional analysis of zebrafish hematopoiesis.
Authors: Konno, Katsuhiro and Kulkeaw, Kasem and Sasada, Manabu and Nii, Takenobu and Kaneyuki, Ayako and Ishitani, Tohru and Arai, Fumio and Sugiyama, Daisuke
Journal: Genes to cells : devoted to molecular & cellular mechanisms (2020): 770-781

[The Establishment of a Three-color Flow Cytometry Approach for the Scoring of Micronucleated Reticulocytes in Rat Bone Marrow].
Authors: Zeng, Zhu and Zhu, Xue-Jiao and Huo, Jiao and Liu, Yun-Jie and Peng, Zi-Hao and Chen, Jin-Yao and Zhang, Li-Shi
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 67-73

TEM observation of compacted DNA of Synechococcus elongatus PCC 7942 using DRAQ5 labelling with DAB photooxidation and osmium black.
Authors: Ghosh, Ilika and Atsuzawa, Kimie and Arai, Aoi and Ohmukai, Ryuzo and Kaneko, Yasuko
Journal: Microscopy (Oxford, England) (2020)

Identification of Cancer-Associated Circulating Cells in Anal Cancer Patients.
Authors: Carter, Thomas J and Jeyaneethi, Jeyarooban and Kumar, Juhi and Karteris, Emmanouil and Glynne-Jones, Rob and Hall, Marcia
Journal: Cancers (2020)

Analysis of erythroid maturation in the nonlysed bone marrow with help of radar plots facilitates detection of flow cytometric aberrations in myelodysplastic syndromes.
Authors: Violidaki, Despoina and Axler, Olof and Jafari, Katayoon and Bild, Filippa and Nilsson, Lars and Mazur, Joanna and Ehinger, Mats and Porwit, Anna
Journal: Cytometry. Part B, Clinical cytometry (2020): 399-411

Influence of Polymer Charge on the Localization and Dark- and Photo-Induced Toxicity of a Potential Type I Photosensitizer in Cancer Cell Models.
Authors: Lindgren, Mikael and Gederaas, Odrun A and Siksjø, Monica and Hansen, Tom A and Chen, Lena and Mettra, Bastien and Andraud, Chantal and Monnereau, Cyrille
Journal: Molecules (Basel, Switzerland) (2020)

Automated cell segmentation in FIJI® using the DRAQ5 nuclear dye.
Authors: Schwendy, Mischa and Unger, Ronald E and Bonn, Mischa and Parekh, Sapun H
Journal: BMC bioinformatics (2019): 39