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Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*
The long-wavelength tracer nuclear yellow is often combined with the popular retrograde tracer true blue for two-color neuronal mapping. In neuronal cells, nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas true blue is a UV light-excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both nuclear yellow and true blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product. Bisbenzimide and Nuclear Yellow, when transported retrogradely through axons to their parent cell bodies, may migrate out of the axons and the cell bodies, as indicated by fluorescence of adjoining glial nuclei. This migration was found to take place both in vivo and in vitro during storage of the sections in water. When using the tracers in 1% concentrations the in vivo migration may be controlled by restricting the survival time. The in vitro migration may be prevented by rapid histological processing of the material.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 167.515 µL | 837.577 µL | 1.675 mL | 8.376 mL | 16.752 mL |
| 5 mM | 33.503 µL | 167.515 µL | 335.031 µL | 1.675 mL | 3.35 mL |
| 10 mM | 16.752 µL | 83.758 µL | 167.515 µL | 837.577 µL | 1.675 mL |
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Citations
View all 2 citations: Citation Explorer
Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations
Authors: Alike, Yamuhanmode and Yushan, Maimaiaili and Keremu, Ajimu and Abulaiti, Alimujiang and Liu, Zhen-Hui and Fu, Wei and Yan, Li-Wei and Yusufu, Aihemaitijiang and Zhu, Qing-Tang and others, undefined
Journal: Neural Regeneration Research (2019): 2173
Authors: Alike, Yamuhanmode and Yushan, Maimaiaili and Keremu, Ajimu and Abulaiti, Alimujiang and Liu, Zhen-Hui and Fu, Wei and Yan, Li-Wei and Yusufu, Aihemaitijiang and Zhu, Qing-Tang and others, undefined
Journal: Neural Regeneration Research (2019): 2173
Design, synthesis, and pharmacological evaluation of second-generation tetrahydroisoquinoline-based CXCR4 antagonists with favorable ADME properties
Authors: Nguyen, Huy H and Kim, Michelle B and Wilson, Robert J and Butch, Christopher J and Kuo, Katie M and Miller, Eric J and Tahirovic, Yesim A and Jecs, Edgars and Truax, Valarie M and Wang, Tao and others,
Journal: Journal of medicinal chemistry (2018): 7168--7188
Authors: Nguyen, Huy H and Kim, Michelle B and Wilson, Robert J and Butch, Christopher J and Kuo, Katie M and Miller, Eric J and Tahirovic, Yesim A and Jecs, Edgars and Truax, Valarie M and Wang, Tao and others,
Journal: Journal of medicinal chemistry (2018): 7168--7188
References
View all 12 references: Citation Explorer
Nuclear death in living Tetrahymena: the case of the haploid nuclei
Authors: Santos ML, Lu E, Wolfe J.
Journal: J Eukaryot Microbiol (2000): 493
Authors: Santos ML, Lu E, Wolfe J.
Journal: J Eukaryot Microbiol (2000): 493
Specific heterochromatic banding of metaphase chromosomes using nuclear yellow
Authors: Pinna-Senn E, Lisanti JA, Ortiz MI, Dalmasso G, Bella JL, Gosalvez J, Stockert JC.
Journal: Biotech Histochem (2000): 132
Authors: Pinna-Senn E, Lisanti JA, Ortiz MI, Dalmasso G, Bella JL, Gosalvez J, Stockert JC.
Journal: Biotech Histochem (2000): 132
Nerve center of the rat extraocular muscles as studied by fluorescent dyes. II: Double labeling study with fast blue and nuclear yellow
Authors: Oda Y., undefined
Journal: Okajimas Folia Anat Jpn (1986): 141
Authors: Oda Y., undefined
Journal: Okajimas Folia Anat Jpn (1986): 141
Projections to the supraoptic and paraventricular nuclei studied in the rat by means of retrograde fluorescent tracers (fast blue, nuclear yellow)
Authors: Stanzani S, Russo A, Leanza G.
Journal: Boll Soc Ital Biol Sper (1984): 2339
Authors: Stanzani S, Russo A, Leanza G.
Journal: Boll Soc Ital Biol Sper (1984): 2339
Afferent and efferent innervation of the rat esophagus. A tracing study with horseradish peroxidase and nuclear yellow
Authors: Fryscak T, Zenker W, Kantner D.
Journal: Anat Embryol (Berl) (1984): 63
Authors: Fryscak T, Zenker W, Kantner D.
Journal: Anat Embryol (Berl) (1984): 63
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