Name: Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*
Brand: AAT Bioquest
SKU: 17539
Price: 232 USD
Availability: InStock

Nuclear Yellow

Hoechst S769121; CAS 74681-68-8

The long-wavelength tracer nuclear yellow is often combined with the popular retrograde tracer true blue for two-color neuronal mapping. In neuronal cells, nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas true blue is a UV light-excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both nuclear yellow and true blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product. Bisbenzimide and Nuclear Yellow, when transported retrogradely through axons to their parent cell bodies, may migrate out of the axons and the cell bodies, as indicated by fluorescence of adjoining glial nuclei. This migration was found to take place both in vivo and in vitro during storage of the sections in water. When using the tracers in 1% concentrations the in vivo migration may be controlled by restricting the survival time. The in vitro migration may be prevented by rapid histological processing of the material.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17539	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	167.515 µL	837.577 µL	1.675 mL	8.376 mL	16.752 mL
5 mM	33.503 µL	167.515 µL	335.031 µL	1.675 mL	3.35 mL
10 mM	16.752 µL	83.758 µL	167.515 µL	837.577 µL	1.675 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	596.96 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations

Authors:

Alike, Yamuhanmode and Yushan, Maimaiaili and Keremu, Ajimu and Abulaiti, Alimujiang and Liu, Zhen-Hui and Fu, Wei and Yan, Li-Wei and Yusufu, Aihemaitijiang and Zhu, Qing-Tang and others, undefined

Journal:

Neural Regeneration Research (2019): 2173

Design, synthesis, and pharmacological evaluation of second-generation tetrahydroisoquinoline-based CXCR4 antagonists with favorable ADME properties

Authors:

Nguyen, Huy H and Kim, Michelle B and Wilson, Robert J and Butch, Christopher J and Kuo, Katie M and Miller, Eric J and Tahirovic, Yesim A and Jecs, Edgars and Truax, Valarie M and Wang, Tao and others,

Journal:

Journal of medicinal chemistry (2018): 7168--7188

References

View all 12 references: Citation Explorer

Nuclear death in living Tetrahymena: the case of the haploid nuclei

Authors:

Santos ML, Lu E, Wolfe J.

Journal:

J Eukaryot Microbiol (2000): 493

Specific heterochromatic banding of metaphase chromosomes using nuclear yellow

Authors:

Pinna-Senn E, Lisanti JA, Ortiz MI, Dalmasso G, Bella JL, Gosalvez J, Stockert JC.

Journal:

Biotech Histochem (2000): 132

Nerve center of the rat extraocular muscles as studied by fluorescent dyes. II: Double labeling study with fast blue and nuclear yellow

Authors:

Oda Y., undefined

Journal:

Okajimas Folia Anat Jpn (1986): 141

Projections to the supraoptic and paraventricular nuclei studied in the rat by means of retrograde fluorescent tracers (fast blue, nuclear yellow)

Authors:

Stanzani S, Russo A, Leanza G.

Journal:

Boll Soc Ital Biol Sper (1984): 2339

Afferent and efferent innervation of the rat esophagus. A tracing study with horseradish peroxidase and nuclear yellow

Authors:

Fryscak T, Zenker W, Kantner D.

Journal:

Anat Embryol (Berl) (1984): 63

Physical properties

Molecular weight	596.96
Solvent	DMSO

Spectral properties

Excitation (nm)	372
Emission (nm)	504

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	74681-68-8

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