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Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*

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Physical properties
Molecular weight596.96
Spectral properties
Excitation (nm)372
Emission (nm)504
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
The long-wavelength tracer nuclear yellow is often combined with the popular retrograde tracer true blue for two-color neuronal mapping. In neuronal cells, nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas true blue is a UV light-excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both nuclear yellow and true blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product. Bisbenzimide and Nuclear Yellow, when transported retrogradely through axons to their parent cell bodies, may migrate out of the axons and the cell bodies, as indicated by fluorescence of adjoining glial nuclei. This migration was found to take place both in vivo and in vitro during storage of the sections in water. When using the tracers in 1% concentrations the in vivo migration may be controlled by restricting the survival time. The in vitro migration may be prevented by rapid histological processing of the material.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM167.515 µL837.577 µL1.675 mL8.376 mL16.752 mL
5 mM33.503 µL167.515 µL335.031 µL1.675 mL3.35 mL
10 mM16.752 µL83.758 µL167.515 µL837.577 µL1.675 mL

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Spectral properties

Excitation (nm)372
Emission (nm)504



View all 2 citations: Citation Explorer
Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations
Authors: Alike, Yamuhanmode and Yushan, Maimaiaili and Keremu, Ajimu and Abulaiti, Alimujiang and Liu, Zhen-Hui and Fu, Wei and Yan, Li-Wei and Yusufu, Aihemaitijiang and Zhu, Qing-Tang and others, undefined
Journal: Neural Regeneration Research (2019): 2173
Design, synthesis, and pharmacological evaluation of second-generation tetrahydroisoquinoline-based CXCR4 antagonists with favorable ADME properties
Authors: Nguyen, Huy H and Kim, Michelle B and Wilson, Robert J and Butch, Christopher J and Kuo, Katie M and Miller, Eric J and Tahirovic, Yesim A and Jecs, Edgars and Truax, Valarie M and Wang, Tao and others,
Journal: Journal of medicinal chemistry (2018): 7168--7188


View all 12 references: Citation Explorer
Nuclear death in living Tetrahymena: the case of the haploid nuclei
Authors: Santos ML, Lu E, Wolfe J.
Journal: J Eukaryot Microbiol (2000): 493
Specific heterochromatic banding of metaphase chromosomes using nuclear yellow
Authors: Pinna-Senn E, Lisanti JA, Ortiz MI, Dalmasso G, Bella JL, Gosalvez J, Stockert JC.
Journal: Biotech Histochem (2000): 132
Nerve center of the rat extraocular muscles as studied by fluorescent dyes. II: Double labeling study with fast blue and nuclear yellow
Authors: Oda Y., undefined
Journal: Okajimas Folia Anat Jpn (1986): 141
Projections to the supraoptic and paraventricular nuclei studied in the rat by means of retrograde fluorescent tracers (fast blue, nuclear yellow)
Authors: Stanzani S, Russo A, Leanza G.
Journal: Boll Soc Ital Biol Sper (1984): 2339
Afferent and efferent innervation of the rat esophagus. A tracing study with horseradish peroxidase and nuclear yellow
Authors: Fryscak T, Zenker W, Kantner D.
Journal: Anat Embryol (Berl) (1984): 63
Evaluation of five basic fluorochromes of potential use in microfluorometric studies of nucleic acids
Authors: Curtis SK, Cowden RR.
Journal: Histochemistry (1983): 503
Simultaneous visualization of Nuclear yellow and iron-dextran complex for demonstration of branched neurons by retrograde axonal transport
Authors: Pollin B, Laplante S, Cesaro P, Nguyen-Legros J.
Journal: J Neurosci Methods (1983): 205
Analysis of phytoplankton by flow cytometry
Authors: Trask BJ, van den Engh GJ, Elgershuizen JH.
Journal: Cytometry (1982): 258
Orthograde transport of Nuclear yellow: a problem and its solution
Authors: Arbuthnott GW, Wright AK, Hamilton MH, Brown JR.
Journal: J Neurosci Methods (1982): 365
Simultaneous visualization of horseradish peroxidase and Nuclear Yellow in tissue sections for neuronal double labeling
Authors: Katan S, Gottschall J, Neuhuber W.
Journal: Neurosci Lett (1982): 121