Calculator
OG488 maleimide [equivalent to Oregon Green® 488 maleimide]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 463.35 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.12 |
| Extinction coefficient (cm -1 M -1) | 76000 |
| Excitation (nm) | 498 |
| Emission (nm) | 526 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Fluoresceins
Molecular weight 463.35 | Correction Factor (260 nm) 0.31 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 76000 | Excitation (nm) 498 | Emission (nm) 526 |
OG488 maleimide is the same molecule to Oregon Green® 488 maleimide (Invitrogen #O6034, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while fluorescein maleimide has pKa ~6.4. The decreased pKa of OG488 maleimide makes its fluorescence essentially pH insensitive in the physiological pH range. This thiol-reactive OG488 maleimide can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to fluorescein maleimide that has severe pH dependence and low photostability.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of OG488 maleimide [equivalent to Oregon Green® 488 maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 215.82 µL | 1.079 mL | 2.158 mL | 10.791 mL | 21.582 mL |
| 5 mM | 43.164 µL | 215.82 µL | 431.639 µL | 2.158 mL | 4.316 mL |
| 10 mM | 21.582 µL | 107.91 µL | 215.82 µL | 1.079 mL | 2.158 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.12 |
| Extinction coefficient (cm -1 M -1) | 76000 |
| Excitation (nm) | 498 |
| Emission (nm) | 526 |
Images
Figure 1. Chemical structure for OG488 maleimide [equivalent to Oregon Green® 488 maleimide]
Citations
View all 1 citations: Citation Explorer
Development of cell-penetration PG-surfactants and its application in external peptide delivery to cytosol
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
Authors: Sumito, Natsumi and Koeda, Shuhei and Umezawa, Naoki and Inoue, Yasumichi and Tsukiji, Shinya and Higuchi, Tsunehiko and Mizuno, Toshihisa
Journal: Bioconjugate Chemistry (2020): 821--833
References
View all 14 references: Citation Explorer
Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654
The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Z and voort M., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Authors: Cheng KT, Van Z and voort M., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Cetuximab-Oregon Green 488
Authors: , undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)
Authors: , undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)
Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alex and er Ross JB., undefined
Journal: Anal Biochem (2002): 18
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alex and er Ross JB., undefined
Journal: Anal Biochem (2002): 18
Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
Fluorescent Oligonucleotide Labeling Reagents