Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>
Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>
logo
AAT Bioquest

Oxazine 1 *CAS 24796-94-9*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight423.89
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)123000
Excitation (nm)648
Emission (nm)668
Quantum yield0.11001
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
24796-94-9
Molecular weight
423.89
Extinction coefficient (cm -1 M -1)
123000
Excitation (nm)
648
Emission (nm)
668
Quantum yield
0.11001
Oxazine 1 is one of the most fluorescent NIR dyes. It has maximum absorption around ~650 nm with extinction coefficient larger than 100,000 M-1 cm-1. It has fluorescence emission around 670 nm, which is pH-independent. Although Oxazine 1 is quite stable from pH 4 to pH 10, it might be reduced by some biological molecules (such as DTT, GSH etc) at high concentration. Oxazine 1 is also an efficient laser dye tunable around 670 nm.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Oxazine 1 *CAS 24796-94-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM235.91 µL1.18 mL2.359 mL11.796 mL23.591 mL
5 mM47.182 µL235.91 µL471.821 µL2.359 mL4.718 mL
10 mM23.591 µL117.955 µL235.91 µL1.18 mL2.359 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)123000
Excitation (nm)648
Emission (nm)668
Quantum yield0.11001

Images


Citations


View all 3 citations: Citation Explorer
Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay
Authors: Zheng, Zhe and Ren, Siying and Geng, Wen-Chao and Cui, Xuexian and Wu, Bian and Wang, Hong
Journal: Chemistry--An Asian Journal (2022): e202200106
A general method to improve fluorophores for live-cell and single-molecule microscopy
Authors: Grimm, Jonathan B and English, Brian P and Chen, Jiji and Slaughter, Joel P and Zhang, Zhengjian and Revyakin, Andrey and Patel, Ronak and Macklin, John J and Normanno, Davide and Singer, Robert H and others, undefined
Journal: Nature methods (2015): 244--250

References


View all 11 references: Citation Explorer
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy
Authors: Vogelsang J, Cordes T, Forthmann C, Steinhauer C, Tinnefeld P.
Journal: Proc Natl Acad Sci U S A (2009): 8107
Effects of pH and electrolyte concentration on the binding between a humic acid and an oxazine dye
Authors: Zanini GP, Avena MJ, Fiol S, Arce F.
Journal: Chemosphere (2006): 430
Analysis of wave packet motion in frequency and time domain: oxazine 1
Authors: Braun M, Sobotta C, Durr R, Pulvermacher H, Malkmus S.
Journal: J Phys Chem A (2006): 9793
In vivo detection of amyloid-beta deposits by near-infrared imaging using an oxazine-derivative probe
Authors: Hintersteiner M, Enz A, Frey P, Jaton AL, Kinzy W, Kneuer R, Neumann U, Rudin M, Staufenbiel M, Stoeckli M, Wiederhold KH, Gremlich HU.
Journal: Nat Biotechnol (2005): 577
Oxazine 170 induces DNA:RNA:DNA triplex formation
Authors: Song G, Xing F, Qu X, Chaires JB, Ren J.
Journal: J Med Chem (2005): 3471
Chirp dependence of wave packet motion in oxazine 1
Authors: Malkmus S, Durr R, Sobotta C, Pulvermacher H, Zinth W, Braun M.
Journal: J Phys Chem A (2005): 10488
Cytochrome P450 modification by a new oxazine derivative with hypolipidemic activity
Authors: Chrysselis MC, Rekka EA, Kourounakis PN.
Journal: Res Commun Mol Pathol Pharmacol (2000): 325
Identification of neutrophils with an oxazine dye
Authors: Kass L., undefined
Journal: Am J Clin Pathol (1987): 436
Flow cytometry of DNA content using oxazine 750 or related laser dyes with 633 nm excitation
Authors: Shapiro HM, Stephens S.
Journal: Cytometry (1986): 107
Oxazine dyes. IV. Simultaneous nuclear and double-contrast cytoplasmic staining from a single solution
Authors: Nathan MJ, Gray P.
Journal: Stain Technol (1961): 237