OxiVision™ Green hydrogen peroxide sensor

Despite the importance of H2O2 to human health and disease, the molecular mechanisms of its production, accumulation, trafficking, and function are insufficiently understood due to the lack of sensitive and specific H2O2 sensors that can be used in live cells. The limitations of currently available H2O2-responsive probes include interfering background fluorescence from other ROS, the need for an external activating enzyme, lack of water solubility or compatibility, and/or excitation profiles in the ultraviolet region. OxiVision Green™ hydrogen peroxide sensor is non-fluorescent and displays no absorption in the visible region. The addition of H2O2 triggers a prompt fluorescence increase with concomitant growth of a visible wavelength absorption band. This probe has a large dynamic range owing to its binary absorption/emission response. The fluorescence response of OxiVision Green™ hydrogen peroxide sensor is H2O2-selective. OxiVision Green™ hydrogen peroxide sensor exhibits a >100-fold selectivity for H2O2 over similar ROS such as O2-, NO, or -OCl.

Fluorescence responses of OxiVision Green™ to various ROS species.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21505	1 mg	Price

Citations

View all 17 citations: Citation Explorer

Chanoclavine synthase operates by an NADPH-independent superoxide mechanism

Authors:

Chen, Chun-Chi and Yu, Zhi-Pu and Liu, Ziwei and Yao, Yongpeng and Hagedoorn, Peter-Leon and Schmitz, Rob Alexander and Yang, Lujia and Yu, Lu and Liu, Aokun and Sheng, Xiang and others,

Journal:

Nature (2025): 1--7

SOD1 Is an Integral Yet Insufficient Oxidizer of Hydrogen Sulfide in Trisomy 21 B Lymphocytes and Can Be Augmented by a Pleiotropic Carbon Nanozyme

Authors:

Mouli, Karthik and Liopo, Anton V and Suva, Larry J and Olson, Kenneth R and McHugh, Emily A and Tour, James M and Derry, Paul J and Kent, Thomas A

Journal:

Antioxidants (2024): 1361

Exosome-Coated Prussian Blue Nanoparticles for Specific Targeting and Treatment of Glioblastoma

Authors:

Hill, Meghan L and Chung, Seock-Jin and Woo, Hyun-Joo and Park, Cho Rong and Hadrick, Kay and Nafiujjaman, Md and Kumar, Panangattukara Prabhakaran Praveen and Mwangi, Leila and Parikh, Rachna and Kim, Taeho

Journal:

ACS Applied Materials \& Interfaces (2024)

Selenite selectively kills lung fibroblasts to treat bleomycin-induced pulmonary fibrosis

Authors:

Lin, Jiun-Han and Liu, Chen-Chi and Liu, Chao-Yu and Hsu, Tien-Wei and Yeh, Yi-Chen and How, Chorng-Kung and Hsu, Han-Shui and Hung, Shih-Chieh

Journal:

Redox Biology (2024): 103148

Rational design of ICD-inducing nanoparticles for cancer immunotherapy

Authors:

Zhang, Zhanzhan and Pan, Zheng and Li, Qiushi and Huang, Qingqing and Shi, Linqi and Liu, Yang

Journal:

Science Advances (2024): eadk0716

References

View all 54 references: Citation Explorer

Genetically encoded fluorescent indicator for intracellular hydrogen peroxide

Authors:

Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S.

Journal:

Nat Methods (2006): 281

Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes

Authors:

Bienert GP, Moller AL, Kristiansen KA, Schulz A, Moller IM, Schjoerring JK, Jahn TP.

Journal:

J Biol Chem. (2006)

Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system

Authors:

Almeida LE, Imasato H, Tabak M.

Journal:

Biochim Biophys Acta (2006): 216

Fluorescent quenching method for determination of trace hydrogen peroxide in rain water

Authors:

Chen H, Yu H, Zhou Y, Wang L.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc. (2006)

Nonenzymatic direct assay of hydrogen peroxide at neutral pH using the Eu3Tc fluorescent probe

Authors:

Durkop A, Wolfbeis OS.

Journal:

J Fluoresc (2005): 755

Physical properties

Molecular weight	~600
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 15, 2025