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OxiVision™ Green hydrogen peroxide sensor

Fluorescence responses of OxiVision Green™ to various ROS species.
Fluorescence responses of OxiVision Green™ to various ROS species.
Fluorescence responses of OxiVision Green™ to various ROS species.
Detection of hydrogen peroxide in Jurkat cells. Cells were stained with OxiVision Green™ peroxide sensor for 30 minutes and treated with 100 µM hydrogen peroxide at 37 °C for 90 minutes. Cells stained with OxiVision Green™ peroxide sensor but without hydrogen peroxide treatment were used as control.
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Physical properties
Molecular weight~600
Spectral properties
Excitation (nm)498
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Despite the importance of H2O2 to human health and disease, the molecular mechanisms of its production, accumulation, trafficking, and function are insufficiently understood due to the lack of sensitive and specific H2O2 sensors that can be used in live cells. The limitations of currently available H2O2-responsive probes include interfering background fluorescence from other ROS, the need for an external activating enzyme, lack of water solubility or compatibility, and/or excitation profiles in the ultraviolet region. OxiVision Green™ hydrogen peroxide sensor is non-fluorescent and displays no absorption in the visible region. The addition of H2O2 triggers a prompt fluorescence increase with concomitant growth of a visible wavelength absorption band. This probe has a large dynamic range owing to its binary absorption/emission response. The fluorescence response of OxiVision Green™ hydrogen peroxide sensor is H2O2-selective. OxiVision Green™ hydrogen peroxide sensor exhibits a >100-fold selectivity for H2O2 over similar ROS such as O2-, NO, or -OCl.


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Spectral properties

Excitation (nm)498
Emission (nm)517



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