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AAT Bioquest

OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor

Product key features

  • Readily used for live cell imaging 
  • Provide high selectivity for mitochondrial lipid peroxidation
  • Compatible with the common Cy3/TRITC filter set
  • Multiplex with other dyes (e.g., DAPI or GFP)

Product description

A wide range of diseases is considered to result from mitochondrial oxidative damage that is associated with the lipid peroxidation of mitochondrial inner membranes. There are no specific methods to assess mitochondrial lipid peroxidation in live cells. To address this unmet need, AAT Bioquest has developed the OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor, a fluorescent mitochondria-targeted probe that detects lipid peroxidation in live cells. OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor enters cells rapidly, and selectively accumulates in mitochondria. It has a high specificity for the detection of mitochondrial lipid peroxidation. Mitochondrial lipid peroxidation results in a great change in fluorescence at 590 nm, which can conveniently be monitored by fluorimetry, fluorescence microscopy or a fluorescence microplate reader.

Example protocol

AT A GLANCE

Important Note

Before initial use, thaw OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor at room temperature and briefly centrifuge to collect the dried pellet.

Protocol Summary
  1. Prepare and treat cells as needed in growth medium

  2. Stain cells with OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution

  3. Incubate samples at 37 °C in a 5% CO₂ incubator for 30–60 minutes

  4. Monitor fluorescence intensity with Cy3 filter

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Protect from light and avoid repeated freeze-thaw cycles

OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor Stock Solution
  1. Prepare 2 to 5 mM stock solution in DMSO. For example, add 20 μL of DMSO into one vial to create a 5 mM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor stock solution.

    Note: Prepare single-use aliquots of the stock solution and store at ≤ -20°C. Protect from light and avoid repeated freeze-thaw cycles.

PREPARATION OF WORKING SOLUTION

OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor Working Solution
  1. Prepare a 500 to 1000 nM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution. For example, add 2 μL of 5 mM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor stock solution to 10 mL of cell culture medium or HHBS buffer (AAT cat# 20011).

    Note: Protect the working solution from light by covering it with foil or placing it in the dark.

    Note: For best results, use the solution within 2 hours of its preparation.

    Note: 10 mL of working solution is enough for 100 tests.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Plate cells as needed in a 96-well black wall, clear bottom plate.

  2. Add treatment to induce lipid peroxidation.

  3. Add 100 µL of OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution to cells.

  4. Incubate cells at 37 ºC in a 5% CO2 incubator for 30–60 minutes, protected from light.

    Note: The optimal concentration and incubation time may vary by cell line; we recommend testing with different concentrations.

  5. Optional: Remove the dye working solution and wash cells twice with HHBS buffer if background fluorescence is observed.

  6. Add HHBS buffer and analyze the cells using a fluorescence microscope with a Cy3 filter set.

References

View all 50 references: Citation Explorer
Mitochondrial lipid peroxidation is necessary but not sufficient for induction of ferroptosis.
Authors: Huan, He and Lyamzaev, Konstantin G and Panteleeva, Alisa A and Chernyak, Boris V
Journal: Frontiers in cell and developmental biology (2024): 1452824
A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression.
Authors: Hai, Yongrui and Fan, Renming and Zhao, Ting and Lin, Ruizhuo and Zhuang, Junyan and Deng, Aohua and Meng, Shanshui and Hou, Zhuang and Wei, Gaofei
Journal: Pharmacological research (2024): 107115
Mitochondrial miR-12294-5p-Regulated Copper Exposure-Caused Mitochondrial-Dependent Ferroptosis by Targeted Inhibition of CISD1 in Chicken Hepatocytes.
Authors: Zhong, Gaolong and Wang, MengRan and Sun, Bingxia and Ma, Feiyang and Yu, Wenlan and Hu, Lianmei and Liao, Jianzhao and Tang, Zhaoxin
Journal: Journal of agricultural and food chemistry (2024): 15948-15958
A platinum(IV)-artesunate complex triggers ferroptosis by boosting cytoplasmic and mitochondrial lipid peroxidation to enhance tumor immunotherapy.
Authors: Fan, Renming and Deng, Aohua and Lin, Ruizhuo and Zhang, Shuo and Cheng, Caiyan and Zhuang, Junyan and Hai, Yongrui and Zhao, Minggao and Yang, Le and Wei, Gaofei
Journal: MedComm (2024): e570
Exogenous Iron Induces Mitochondrial Lipid Peroxidation, Lipofuscin Accumulation, and Ferroptosis in H9c2 Cardiomyocytes.
Authors: Lyamzaev, Konstantin G and Huan, He and Panteleeva, Alisa A and Simonyan, Ruben A and Avetisyan, Armine V and Chernyak, Boris V
Journal: Biomolecules (2024)
Page updated on December 13, 2024

Ordering information

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Catalog Number21510
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

843.20

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

Fluorescence microscope

ExcitationCy3 Filter
EmissionCy3 Filter
Recommended plateBlack wall, clear bottom
Fluorescence response of OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor (1000 nM) in HeLa cells with or without Erastin (10 µM) treatment at 37 ºC in a 5% CO2 incubator for 30 minutes. The fluorescence intensities were monitored with fluorescence microscopy using Cy3 filter.
Fluorescence response of OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor (1000 nM) in HeLa cells with or without Erastin (10 µM) treatment at 37 ºC in a 5% CO2 incubator for 30 minutes. The fluorescence intensities were monitored with fluorescence microscopy using Cy3 filter.
Fluorescence response of OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor (1000 nM) in HeLa cells with or without Erastin (10 µM) treatment at 37 ºC in a 5% CO2 incubator for 30 minutes. The fluorescence intensities were monitored with fluorescence microscopy using Cy3 filter.