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Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol]
Oxonol V is a sensitive slow-response membrane potential probe that is widely used for measuring membrane potentials of many biological systems. The fluorescence of Oxonol V decreases upon membrane hyperpolarization. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 260.152 µL | 1.301 mL | 2.602 mL | 13.008 mL | 26.015 mL |
| 5 mM | 52.03 µL | 260.152 µL | 520.305 µL | 2.602 mL | 5.203 mL |
| 10 mM | 26.015 µL | 130.076 µL | 260.152 µL | 1.301 mL | 2.602 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 27 references: Citation Explorer
Use of an oxonol dye in combination with confocal laser scanning microscopy to monitor damage to Staphylococcus aureus cells during colonisation of silver-coated vascular grafts
Authors: Strathmann M, Wingender J.
Journal: Int J Antimicrob Agents (2004): 234
Authors: Strathmann M, Wingender J.
Journal: Int J Antimicrob Agents (2004): 234
Rapid assessment of antibiotic effects on Escherichia coli by bis-(1,3-dibutylbarbituric acid) trimethine oxonol and flow cytometry
Authors: Jepras RI, Paul FE, Pearson SC, Wilkinson MJ.
Journal: Antimicrob Agents Chemother (1997): 2001
Authors: Jepras RI, Paul FE, Pearson SC, Wilkinson MJ.
Journal: Antimicrob Agents Chemother (1997): 2001
Estimation of membrane potential deltapsi in reconstituted plasma membrane vesicles using a numerical model of oxonol VI distribution
Authors: Portele A, Lenz J, Hofer M.
Journal: J Bioenerg Biomembr (1997): 603
Authors: Portele A, Lenz J, Hofer M.
Journal: J Bioenerg Biomembr (1997): 603
Membrane potential changes visualized in complete growth media through confocal laser scanning microscopy of bis-oxonol-loaded cells
Authors: Dall'Asta V, Gatti R, Orl and ini G, Rossi PA, Rotoli BM, Sala R, Bussolati O, Gazzola GC.
Journal: Exp Cell Res (1997): 260
Authors: Dall'Asta V, Gatti R, Orl and ini G, Rossi PA, Rotoli BM, Sala R, Bussolati O, Gazzola GC.
Journal: Exp Cell Res (1997): 260
Muscarinic depolarization of SH-SY5Y human neuroblastoma cells as determined using oxonol V
Authors: Kukkonen JP, Hautala R, Akerman KE.
Journal: Neurosci Lett (1996): 57
Authors: Kukkonen JP, Hautala R, Akerman KE.
Journal: Neurosci Lett (1996): 57
Page updated on July 12, 2023
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