PacBlue succinimidyl ester [equivalent to Pacific Blue succinimidyl ester]

PacBlue succinimidyl ester

equivalent to Pacific Blue succinimidyl ester

PacBlue succinimidyl ester is the same molecule to Pacific Blue™ succinimidyl ester (Pacific Blue™ is the trademark of ThermoFisher). The amine-reactive Pac Blue succinimidyl ester can be used to prepare the blue fluorescent antibody conjugates that can be well excited by the 405 nm spectral line of the blue diode (violet) laser. These antibody conjugates are widely used in flow cytometry applications.

Flow cytometry analysis of PBMC stained with PacBlue anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PacBlue specific V3-A channel.

Catalog	Size	Price	Quantity
570	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of PacBlue succinimidyl ester [equivalent to Pacific Blue succinimidyl ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	294.803 µL	1.474 mL	2.948 mL	14.74 mL	29.48 mL
5 mM	58.961 µL	294.803 µL	589.605 µL	2.948 mL	5.896 mL
10 mM	29.48 µL	147.401 µL	294.803 µL	1.474 mL	2.948 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	339.21 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	339.21
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.15
Correction factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	215868-33-0

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Page updated on September 25, 2025