PE-iFluor® 594 Tandem
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~240000 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 566 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 606 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS), PE and APC, Spectral Flow Cytometry
Molecular weight ~240000 | Absorbance (nm) 566 | Extinction coefficient (cm -1 M -1) 1960000 | Excitation (nm) 565 | Emission (nm) 606 |
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 594 is a superior replacement to the commonly used PE-Texas Red tandem with significantly improved FRET efficiency and signal/background ratio. Its primary absorption peak is at 565 nm with emission peak around 610 nm. AAT Bioquest also offers a unique preactivated PE-iFluor® 594 to facilitate the PE- iFluor® 594 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 594 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE tandems are conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 566 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 606 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
PE-iFluor™ 597 Tandem | 565 | 612 | - |
PE-iFluor™ 740 Tandem | 565 | 767 | 1960000 |
PE-iFluor™ 720 Tandem | 565 | 750 | 1960000 |
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Images

Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE/iFluor® 594 specific B6-A channel.

Figure 2. Stain index comparison of CD4+ signal using fluorophore-labeled antibody conjugates. Human peripheral blood mononuclear cells (PBMCs) were isolated and stained using AAT Bioquest PE/iFluor® 594 anti-human CD4 conjugates or Biolegend PE/Dazzle™ 594 anti-human CD conjugates. The fluorescence signal was monitored using an Aurora flow cytometer in the PE/iFluor® 594 specific B6-A channel.
References
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