PE-iFluor® 594 Tandem
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~240000 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 566 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 606 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight ~240000 | Absorbance (nm) 566 | Extinction coefficient (cm -1 M -1) 1960000 | Excitation (nm) 565 | Emission (nm) 606 |
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 594 is a superior replacement to the commonly used PE-Texas Red tandem with significantly improved FRET efficiency and signal/background ratio. Its primary absorption peak is at 565 nm with emission peak around 610 nm. AAT Bioquest also offers a unique preactivated PE-iFluor® 594 to facilitate the PE- iFluor® 594 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 594 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE tandems are conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 566 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 606 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
PE-iFluor® 597 Tandem | 565 | 612 | - |
PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
Show More (2) |
Images
Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
Figure 2. Stain index comparison of CD4+ signal using fluorophore-labeled antibody conjugates. Human peripheral blood mononuclear cells (PBMCs) were isolated and stained using AAT Bioquest PE/iFluor® 594 anti-human CD4 conjugates or Biolegend PE/Dazzle™ 594 anti-human CD conjugates. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
References
View all 8 references: Citation Explorer
Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity.
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Optimization of three- and four-color multiparameter DNA analysis in lymphoma specimens.
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Conjugation of fluorochromes to monoclonal antibodies.
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
A strategy for multiple immunophenotyping by image cytometry: model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes.
Authors: Gothot, A and Grosdent, J C and Paulus, J M
Journal: Cytometry (1996): 214-25
Authors: Gothot, A and Grosdent, J C and Paulus, J M
Journal: Cytometry (1996): 214-25
CUBIC: a three-dimensional colored projection of Consort 30 generated trivariate flow cytometric data.
Authors: Greimers, R and Rongy, A M and Schaaf-Lafontaine, N and Boniver, J
Journal: Cytometry (1991): 570-8
Authors: Greimers, R and Rongy, A M and Schaaf-Lafontaine, N and Boniver, J
Journal: Cytometry (1991): 570-8
Concomitant delineation of surface Ig, B-cell differentiation antigens, and HLADR on lymphoid proliferations using three-color immunocytometry.
Authors: Segal, G H and Edinger, M G and Owen, M and McNealis, M and Lopez, P and Perkins, A and Linden, M D and Fishleder, A J and Stoler, M H and Tubbs, R R
Journal: Cytometry (1991): 350-9
Authors: Segal, G H and Edinger, M G and Owen, M and McNealis, M and Lopez, P and Perkins, A and Linden, M D and Fishleder, A J and Stoler, M H and Tubbs, R R
Journal: Cytometry (1991): 350-9
Application notes
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