PE-iFluor® 594 is a high-performance tandem fluorophore optimized for flow cytometry, immunophenotyping, and fluorescence-activated cell sorting (FACS). Upon covalent attachment to antibodies or proteins via maleimide-activated crosslinkers such as SMCC, it enables precise detection of cellular markers in complex, multicolor panels.

This tandem dye combines R-phycoerythrin (PE) as the donor and iFluor® 594 as the acceptor, yielding a bright, photostable emission profile with excitation and emission maxima at 565 nm and 606 nm, respectively. PE-iFluor® 594 is efficiently excited by both 488 nm and 561 nm laser lines and is compatible with standard 610/20 nm bandpass filters, facilitating integration into conventional cytometry systems.

Compared to traditional PE-Texas Red conjugates, PE-iFluor® 594 offers enhanced fluorescence intensity, improved quantum yield, and lower background, resulting in superior signal-to-noise ratios and reduced spectral overlap—critical parameters for high-dimensional cytometry. Its robust photostability ensures consistent performance during extended acquisition, enabling accurate detection of low-abundance targets and rare cell populations.

For added convenience, PE-iFluor® 594 is also available in a ReadiUse® preactivated format. Pre-functionalized with Buccutite® FOL chemistry, it enables efficient, thiol-free conjugation. In this system, the target antibody or protein is first modified with Buccutite® MTA, then reacted with the FOL-activated PE-iFluor® 594, producing stable, bioactive conjugates in a rapid, aqueous workflow, ready for immediate use in flow cytometry applications.