PE-iFluor® 610 Tandem

PE-iFluor® 610 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~630 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 610 Tandem gives a significantly distinct color compared to the commonly used PE-Texas Red Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE/iFluor® 610 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 610 specific B6-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2700	1 mg	Price

References

View all 8 references: Citation Explorer

Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.

Authors:

Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule

Journal:

Clinical nutrition (Edinburgh, Scotland) (2014): 130-5

Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity.

Authors:

Fromm, Jonathan R and Thomas, Anju and Wood, Brent L

Journal:

American journal of clinical pathology (2009): 322-32

Optimization of three- and four-color multiparameter DNA analysis in lymphoma specimens.

Authors:

Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74

Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.

Authors:

Mathioudakis, George and Coder, David and Fefer, Alexander

Journal:

Journal of immunological methods (2002): 37-42

Conjugation of fluorochromes to monoclonal antibodies.

Authors:

Holmes, K L and Lantz, L M and Russ, W

Journal:

Current protocols in cytometry (2001): Unit 4.2

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	565
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	625

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Labeling Antibodies & Biopolymers
Brochure: PE & APC Tandem Dyes

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Fax
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Page updated on October 28, 2025