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PE-iFluor® 610 Tandem

Documents
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Related catalogs
Fluorescence Activated Cell Sorting (FACS)
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Spectral Flow Cytometry
FAQ
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Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Buccutite™ Bioconjugation Technology
AssayWise
HRP Antibody Labeling Using Buccutite™ Crosslinking Technology
iFluor® 700 Dyes
Hydroxyl Radical Detection
Buccutite™ Conjugation Kits: Quick and Easy Antibody Labeling
Nucleic Acid Detection, Quantification and Imaging
PE-iFluor® 610 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~630 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 610 Tandem gives a significantly distinct color compared to the commonly used PE-Texas Red Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE/iFluor® 610 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 610 specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE/iFluor® 610 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 610 specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE/iFluor® 610 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 610 specific B6-A channel.
Ordering information
Price
Unit size
Catalog Number2700
Quantity
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Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Physical properties
SolventWater
Spectral properties
Absorbance (nm)565
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)625
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
Show More (2)
References
View all 8 references: Citation Explorer
Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity.
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Optimization of three- and four-color multiparameter DNA analysis in lymphoma specimens.
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Conjugation of fluorochromes to monoclonal antibodies.
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
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