PE-iFluor® 647 Tandem

Flow cytometry analysis of PBMC stained with PE-iFluor® 647 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 647 specific B8-A channel.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	666

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

666

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 647 is a superior replacement to the commonly used PE-Cy5 tandem with significantly improved FRET efficiency and signal/background ratio. PE-iFluor® 647 and PE-Cy5® and RPE-Alexa Fluor® 647 Tandem have almost identical spectra. AAT Bioquest also offers a unique preactivated PE-iFluor® 647 to facilitate the PE- iFluor® 647 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 647 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	666

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
PE-iFluor® 700 Tandem	565	708	1960000
PE-iFluor® 610 Tandem	565	625	1960000
PE-iFluor® 750 Tandem	565	778	1960000
PE-iFluor® 710 Tandem	565	747	1960000
PE-iFluor® 594 Tandem	565	606	1960000
PE-iFluor® 660 Tandem	565	695	1960000
PE-iFluor™ 597 Tandem	565	612	-
PE-iFluor™ 740 Tandem	565	767	1960000
PE-iFluor™ 720 Tandem	565	750	1960000
PE-iFluor™ 770 Tandem	565	795	1960000
Show More (1)

Images

Figure 1. Flow cytometry analysis of PBMC stained with PE-iFluor® 647 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 647 specific B8-A channel.

References

View all 49 references: Citation Explorer

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Authors: Kowalska, Wioleta
Journal: Folia histochemica et cytobiologica (2020): 17-24

Anti-epithelial cell adhesion molecule RNA aptamer-conjugated liposomal doxorubicin as an efficient targeted therapy in mice bearing colon carcinoma tumor model.
Authors: Mashreghi, Mohammad and Zamani, Parvin and Karimi, Maryam and Mehrabian, Amin and Arabsalmani, Mahdieh and Zarqi, Javad and Moosavian, Seyedeh Alia and Jaafari, Mahmoud Reza
Journal: Biotechnology progress (2020): e3116

Image cytometry as an alternative to flow cytometry for the transplant histocompatibility crossmatch assay.
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Journal: HLA (2019): 436-444

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Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713

Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1.
Authors: Khamechian, Tahereh and Irandoust, Behnaz and Mohammadi, Hanieh and Nikoueinejad, Hassan and Akbari, Hossein
Journal: Iranian journal of allergy, asthma, and immunology (2018): 151-157

A Handy Liquid Metal Based Non-Invasive Electrophoretic Particle Microtrap.
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Journal: Micromachines (2018)

A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080

Multiplexed Lipid Bilayers on Silica Microspheres for Analytical Screening Applications.
Authors: Fernandez Oropeza, Nadiezda and Zurek, Nesia A and Galvan-De La Cruz, Mirella and Fabry-Wood, Aurora and Fetzer, Jennifer M and Graves, Steven W and Shreve, Andrew P
Journal: Analytical chemistry (2017): 6440-6447

Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis.
Authors: Laopajon, Witida and Takheaw, Nuchjira and Kasinrerk, Watchara and Pata, Supansa
Journal: Journal of immunoassay & immunochemistry (2016): 527-39