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PE-iFluor® 647 Tandem
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 647 is a superior replacement to the commonly used PE-Cy5 tandem with significantly improved FRET efficiency and signal/background ratio. PE-iFluor® 647 and PE-Cy5® and RPE-Alexa Fluor® 647 Tandem have almost identical spectra. AAT Bioquest also offers a unique preactivated PE-iFluor® 647 to facilitate the PE- iFluor® 647 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 647 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.
Flow cytometry analysis of PBMC stained with PE-iFluor® 647 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 647 specific B8-A channel.
Flow cytometry analysis of PBMC stained with PE-iFluor® 647 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 647 specific B8-A channel.
CatalogSize
Price
Quantity
27021 mg
Price
 
Physical properties

SolventWater
Spectral properties

Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)666
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 5, 2025