Name: PE-iFluor® 647 Tandem
Brand: AAT Bioquest
SKU: 2702
Price: 237 USD
Availability: InStock

PE-iFluor® 647 Tandem

PE-iFluor® 647 Tandem is a PE-based tandem fluorophore that uses phycoerythrin (PE) as the donor and iFluor® 647 as the acceptor dye for far-red fluorescence detection in flow cytometry.

Detection Principle: Uses fluorescence resonance energy transfer (FRET) between PE and iFluor® 647.
Spectral Properties: Excitation/emission maxima of 565/666 nm.
Enhanced Photostability: Offers improved resistance to photobleaching.
Applications: Suitable for conventional multicolor and spectral flow cytometry.

Flow cytometry analysis of PBMC stained with PE-iFluor® 647 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 647 specific B8-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2702	1 mg	Price

Overview

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 647 is a superior replacement to the commonly used PE-Cy5 tandem with significantly improved FRET efficiency and signal/background ratio. PE-iFluor® 647 and PE-Cy5® and RPE-Alexa Fluor® 647 Tandem have almost identical spectra. AAT Bioquest also offers a unique preactivated PE-iFluor® 647 to facilitate the PE-iFluor® 647 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor® 647 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.

References

View all 49 references: Citation Explorer

Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients.

Authors:

Kowalska, Wioleta

Journal:

Folia histochemica et cytobiologica (2020): 17-24

Anti-epithelial cell adhesion molecule RNA aptamer-conjugated liposomal doxorubicin as an efficient targeted therapy in mice bearing colon carcinoma tumor model.

Authors:

Mashreghi, Mohammad and Zamani, Parvin and Karimi, Maryam and Mehrabian, Amin and Arabsalmani, Mahdieh and Zarqi, Javad and Moosavian, Seyedeh Alia and Jaafari, Mahmoud Reza

Journal:

Biotechnology progress (2020): e3116

Image cytometry as an alternative to flow cytometry for the transplant histocompatibility crossmatch assay.

Authors:

Ramon, Daniel S and Franks, Thomas and Jaramillo, Andrés and Paradis, Benjamin D and Chan, Leo Li-Ying

Journal:

HLA (2019): 436-444

Initial flow cytometric evaluation of the Clearllab lymphoid screen.

Authors:

Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L

Journal:

Cytometry. Part B, Clinical cytometry (2018): 707-713

Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1.

Authors:

Khamechian, Tahereh and Irandoust, Behnaz and Mohammadi, Hanieh and Nikoueinejad, Hassan and Akbari, Hossein

Journal:

Iranian journal of allergy, asthma, and immunology (2018): 151-157

Physical properties

Molecular weight	~240000
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	666

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on November 12, 2026