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PE-iFluor® 647 Tandem
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Application notes
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 647 is a superior replacement to the commonly used PE-Cy5 tandem with significantly improved FRET efficiency and signal/background ratio. PE-iFluor® 647 and PE-Cy5® and RPE-Alexa Fluor® 647 Tandem have almost identical spectra. AAT Bioquest also offers a unique preactivated PE-iFluor® 647 to facilitate the PE- iFluor® 647 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 647 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
| PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
| PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
| PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
| PE-iFluor® 594 Tandem | 565 | 606 | 1960000 |
| PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
| PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
| PE-iFluor® 597 Tandem | 565 | 612 | - |
| PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
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References
View all 49 references: Citation Explorer
Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients.
Authors: Kowalska, Wioleta
Journal: Folia histochemica et cytobiologica (2020): 17-24
Authors: Kowalska, Wioleta
Journal: Folia histochemica et cytobiologica (2020): 17-24
Anti-epithelial cell adhesion molecule RNA aptamer-conjugated liposomal doxorubicin as an efficient targeted therapy in mice bearing colon carcinoma tumor model.
Authors: Mashreghi, Mohammad and Zamani, Parvin and Karimi, Maryam and Mehrabian, Amin and Arabsalmani, Mahdieh and Zarqi, Javad and Moosavian, Seyedeh Alia and Jaafari, Mahmoud Reza
Journal: Biotechnology progress (2020): e3116
Authors: Mashreghi, Mohammad and Zamani, Parvin and Karimi, Maryam and Mehrabian, Amin and Arabsalmani, Mahdieh and Zarqi, Javad and Moosavian, Seyedeh Alia and Jaafari, Mahmoud Reza
Journal: Biotechnology progress (2020): e3116
Image cytometry as an alternative to flow cytometry for the transplant histocompatibility crossmatch assay.
Authors: Ramon, Daniel S and Franks, Thomas and Jaramillo, Andrés and Paradis, Benjamin D and Chan, Leo Li-Ying
Journal: HLA (2019): 436-444
Authors: Ramon, Daniel S and Franks, Thomas and Jaramillo, Andrés and Paradis, Benjamin D and Chan, Leo Li-Ying
Journal: HLA (2019): 436-444
Initial flow cytometric evaluation of the Clearllab lymphoid screen.
Authors: Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L
Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713
Authors: Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L
Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713
Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1.
Authors: Khamechian, Tahereh and Irandoust, Behnaz and Mohammadi, Hanieh and Nikoueinejad, Hassan and Akbari, Hossein
Journal: Iranian journal of allergy, asthma, and immunology (2018): 151-157
Authors: Khamechian, Tahereh and Irandoust, Behnaz and Mohammadi, Hanieh and Nikoueinejad, Hassan and Akbari, Hossein
Journal: Iranian journal of allergy, asthma, and immunology (2018): 151-157