PE-iFluor® 700 Tandem

PE-iFluor® 700 Tandem is a PE-based tandem fluorophore that uses phycoerythrin (PE) as the donor and iFluor® 700 as the acceptor dye for far-red fluorescence detection in flow cytometry.

Detection Principle: Uses fluorescence resonance energy transfer (FRET) between PE and iFluor® 700.
Spectral Properties: Excitation/emission maxima of 565/708 nm.
Enhanced Staining Performance: Provides a significantly higher staining index than PE-Alexa Fluor® 700 Tandem.
Applications: Suitable for conventional multicolor and spectral flow cytometry.

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2614	1 mg	Price

Overview

PE-iFluor® 700 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~720 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 700 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

References

View all 13 references: Citation Explorer

Multifunctional Silica-Based Nanoparticles with Controlled Release of Organotin Metallodrug for Targeted Theranosis of Breast Cancer.

Authors:

Ovejero Paredes, Karina and Díaz-García, Diana and García-Almodóvar, Victoria and Lozano Chamizo, Laura and Marciello, Marzia and Díaz-Sánchez, Miguel and Prashar, Sanjiv and Gómez-Ruiz, Santiago and Filice, Marco

Journal:

Cancers (2020)

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.

Authors:

Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar

Journal:

Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29

[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].

Authors:

Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin

Journal:

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965

Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer.

Authors:

Kessler, Claudia and Pardo, Alessa and Tur, Mehmet K and Gattenlöhner, Stefan and Fischer, Rainer and Kolberg, Katharina and Barth, Stefan

Journal:

Journal of cancer research and clinical oncology (2017): 2025-2038

Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry.

Authors:

Carulli, Giovanni and Marini, Alessandra and Sammuri, Paola and Domenichini, Cristiana and Ottaviano, Virginia and Pacini, Simone and Petrini, Mario

Journal:

Journal of clinical and experimental hematopathology : JCEH (2015): 55-60

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	708

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on November 9, 2026