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PE-iFluor® 700 Tandem

Documents
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Related catalogs
Fluorescence Activated Cell Sorting (FACS)
PE and APC
Spectral Flow Cytometry
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Both unicellular and multicellular organisms undergo mitosis. What are the differences observed in the process between the two?
Can 7-AAD be fixed?
Can 7-AAD stain live cells?
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Buccutite™ Bioconjugation Technology
AssayWise
HRP Antibody Labeling Using Buccutite™ Crosslinking Technology
iFluor® 700 Dyes
Hydroxyl Radical Detection
Buccutite™ Conjugation Kits: Quick and Easy Antibody Labeling
Nucleic Acid Detection, Quantification and Imaging
PE-iFluor® 700 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~720 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 700 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
Flow cytometry analysis of whole blood stained with PE-iFluor® 700 anti-human CD19 *HIB19* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 700 specific B10-A channel.
Ordering information
Price
Unit size
Catalog Number2614
Quantity
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Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
SolventWater
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)708
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
APC-iFluor® 700 Tandem651710700000
Show More (3)
References
View all 13 references: Citation Explorer
Multifunctional Silica-Based Nanoparticles with Controlled Release of Organotin Metallodrug for Targeted Theranosis of Breast Cancer.
Authors: Ovejero Paredes, Karina and Díaz-García, Diana and García-Almodóvar, Victoria and Lozano Chamizo, Laura and Marciello, Marzia and Díaz-Sánchez, Miguel and Prashar, Sanjiv and Gómez-Ruiz, Santiago and Filice, Marco
Journal: Cancers (2020)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer.
Authors: Kessler, Claudia and Pardo, Alessa and Tur, Mehmet K and Gattenlöhner, Stefan and Fischer, Rainer and Kolberg, Katharina and Barth, Stefan
Journal: Journal of cancer research and clinical oncology (2017): 2025-2038
Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry.
Authors: Carulli, Giovanni and Marini, Alessandra and Sammuri, Paola and Domenichini, Cristiana and Ottaviano, Virginia and Pacini, Simone and Petrini, Mario
Journal: Journal of clinical and experimental hematopathology : JCEH (2015): 55-60
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