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PE-iFluor® 700 Tandem
PE-iFluor® 700 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~720 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 700 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 700 specific B10-A channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
26141 mg
Price
 
Physical properties
SolventWater
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)708
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Labeling Antibodies & Biopolymers
Brochure: PE & APC Tandem Dyes
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Page updated on October 31, 2025