PE-iFluor® 700 Tandem

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE/iFluor® 700 specific B10-A channel.

Flow cytometry analysis of whole blood stained with PE-iFluor® 700 anti-human CD19 *HIB19* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 700 specific B10-A channel.

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	708

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Absorbance (nm)

566

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

708

PE-iFluor® 700 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~720 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 700 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	708

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
PE-iFluor® 610 Tandem	565	625	1960000
PE-iFluor® 647 Tandem	565	666	1960000
PE-iFluor® 750 Tandem	565	778	1960000
PE-iFluor® 710 Tandem	565	747	1960000
PE-iFluor® 594 Tandem	565	606	1960000
PE-iFluor® 660 Tandem	565	695	1960000
PE-iFluor™ 597 Tandem	565	612	-
PE-iFluor™ 740 Tandem	565	767	1960000
PE-iFluor™ 720 Tandem	565	750	1960000
PE-iFluor™ 770 Tandem	565	795	1960000
APC-iFluor® 700 Tandem	651	710	700000
Show More (2)

Images

Figure 1. Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE/iFluor® 700 specific B10-A channel.

Figure 2. Flow cytometry analysis of whole blood stained with PE-iFluor® 700 anti-human CD19 *HIB19* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 700 specific B10-A channel.

References

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Multifunctional Silica-Based Nanoparticles with Controlled Release of Organotin Metallodrug for Targeted Theranosis of Breast Cancer.
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Journal: Cancers (2020)

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
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Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry.
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A novel modification of a flow cytometric assay of phosphorylated STAT1 in whole blood lymphocytes for rapid detection of interferon-alpha signal in vivo.
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Journal: Cytometry. Part B, Clinical cytometry (2006): 293-301

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